PMID: 7026686Jan 1, 1981Paper

Novel preparation method of immunogen for hydrophobic hapten, enzyme immunoassay for daunomycin and adriamycin

Journal of Immunological Methods
K FujiwaraT Kitagawa

Abstract

The present study was undertaken to develop a novel method of preparing a hydrophobic hapten as immunogen. The anticancer drug, daunomycin (DM) was used as prototype and coupled to mercaptosuccinylated bovine serum albumin (MS, BSA) with N-(gamma-maleimidobutyryloxy)succinimide (GMBS). Injection of rabbits with a conjugate (DM-GMBS-MS.BSA) which contained 7.3 DM per BSA molecule produced good levels of anti-DM antibody which was detected by the reaction of diluted antiserum with beta-D-galactosidase-labeled DM. beta-D-galactosidase-labeled DM was used to develop a double antibody enzyme immunoassay for DM and for the DM homologue adriamycin (AM) which reproducibly detected as little as 1 pmole of either drug. A variety of commonly used other anticancer drugs were tested and had little reactivity in this immunoassay. These studies indicate that the anti-DM serum produced is highly specific.

References

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Citations

Jul 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·M OhtaK Ohta
Nov 1, 1995·Thrombosis Research·F ManningB Woodhams
May 1, 1992·Molecular Immunology·A AvrameasA D Strosberg
Jul 10, 1999·Bioorganic & Medicinal Chemistry·L GilR N Young
May 20, 2009·Antimicrobial Agents and Chemotherapy·Kunio FujiwaraLars-Inge Larsson
Oct 30, 2007·Journal of Chromatography. a·Samir SafiAgnès Hagège
Mar 12, 2004·Biochemical and Biophysical Research Communications·Young Hye KimYoung-Ho Chung
Apr 6, 2005·Bioorganic & Medicinal Chemistry·Nebojsa IlićVolker Magnus
Nov 24, 2001·Biochimica Et Biophysica Acta·J N MoreiraT M Allen
Apr 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kunio FujiwaraKazuhiro Tsukamoto
May 10, 1985·Journal of Chromatography·S Eksborg, H Ehrsson

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