Abstract
Biohydrogen (H2) production by purple bacteria during photofermentation is a very promising way among biological H2 production methods. The effects of protonophores, carbonyl cyanide m-chlorophenylhydrazone (CCCP), 2,4-dinitrophenol (DNP), and inhibitors of enzymes, involved in H2 metabolism, metronidazole (Met), diphenyleneiodonium (DPI), and dimethylsulphoxide (DMSO) on H2 production by Rhodobacter sphaeroides MDC6522 isolated from Jermuk mineral springs in Armenia have been investigated in both nitrogen-limited and nitrogen-excess conditions. With the increase of inhibitors concentrations H2 yield gradually decreased. The complete inhibition of H2 production was observed in the presence of DPI and CCCP. DPI's solvent-DMSO in low concentration did not significantly affect H2 yield. N,N'-dicyclohexylcarbodiimide (DCCD)-inhibited the FOF1-ATPase activity of bacterial membrane vesicles was analyzed in the presence of inhibitors. Low concentrations of DPI and DMSO did not affect ATPase activity, whereas Met and CCCP stimulated enzyme activity. The effect of DNP was similar to CCCP. The results have shown the low concentration or concentration dependent effects of protonophores and nitrogenase and hydrogenase inhibitors on photofe...Continue Reading
References
Apr 11, 1979·Biochimica Et Biophysica Acta·R M Tetley, N I Bishop
Nov 1, 1977·Applied and Environmental Microbiology·A DadayG D Smith
Jun 1, 1987·Journal of Bacteriology·R L SmithF R Tabita
Jan 1, 1986·Biochemical Pharmacology·M Müller
Sep 2, 1998·Molecular Membrane Biology·Z W Yu, P J Quinn
Sep 3, 1999·The Journal of Biological Chemistry·C WassmannI Bruchhaus
Aug 5, 2000·Biochimica Et Biophysica Acta·P MagnaniT Lissolo
May 25, 2002·Letters in Applied Microbiology·S A MarkarianK A Bagramyan
Jun 14, 2005·Biochimica Et Biophysica Acta·Florence MusGilles Peltier
Dec 24, 2005·Microbial Cell Factories·Debajyoti DuttaSanjoy K Bhattacharya
Apr 21, 2010·PloS One·Ken NoguchiJoan L Slonczewski
Nov 11, 2010·Prikladnaia biokhimiia i mikrobiologiia·Z A El'tsovaA A Tsigankov
Dec 1, 2011·The Journal of Biological Chemistry·Nicholas J SkizimG Charles Dismukes
Feb 9, 2012·Critical Reviews in Biochemistry and Molecular Biology·Karen TrchounianArmen Trchounian
Feb 22, 2012·Bioresource Technology·Patrick C HallenbeckDipankar Ghosh
Jun 13, 2012·Journal of Bioenergetics and Biomembranes·Lilit HakobyanArmen Trchounian
Oct 9, 2012·Biotechnology and Bioengineering·Lisa CariusHartmut Grammel
Apr 20, 2013·Biochimica Et Biophysica Acta·Lance C SeefeldtDennis R Dean
Jul 31, 2013·Critical Reviews in Biotechnology·Armen Trchounian
Oct 12, 2013·FEMS Microbiology Letters·Anna PoladyanArmen Trchounian
Feb 27, 2015·Biofizika·A I KonovalovIu V Kiseleva
Citations
Aug 2, 2016·Journal of Photochemistry and Photobiology. B, Biology·Lilit GabrielyanArmen Trchounian
Oct 26, 2016·Journal of Photochemistry and Photobiology. B, Biology·Lilit GabrielyanArmen Trchounian
Nov 12, 2016·Bioelectrochemistry·Jingya ShenGianluca Li Puma