Novel quartz crystal microbalance based biosensor for detection of oral epithelial cell-microparticle interaction in real-time

Biosensors & Bioelectronics
Jacqueline ElsomA Christy Hunter

Abstract

Recent applications of quartz crystal resonant sensor technology to monitor cell adhesion and specific ligand interaction processes has triggered the development of a new category of quartz crystal microbalance (QCM) based biosensors. In this study human oral epithelial cells (H376) were cultured on quartz sensors and their response to microspheres investigated in situ using the QCM technique. The results demonstrated that this novel biosensor was able to follow cell-microsphere interactions in real-time and under conditions of flow as would occur in the oral cavity. Unique frequency profiles generated in response to the microspheres were postulated to be due to phases of mass addition and altered cellular rigidity. Supporting microscopic evidence demonstrated that the unique frequency responses obtained to these interactions were in part due to binding between the cell surface and the microspheres. Furthermore, a cellular uptake process, in response to microsphere loading was identified and this, by influencing the rigidity of the cellular cytoskeleton, was also detectable through the frequency responses obtained.

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Citations

Mar 26, 2011·Clinical & Developmental Immunology·Lixia ZhouDilan Qin
Oct 15, 2011·Cellular and Molecular Life Sciences : CMLS·Michael Saitakis, Electra Gizeli
Aug 1, 2012·Nanomedicine : Nanotechnology, Biology, and Medicine·A Christy HunterS Moein Moghimi
Jun 7, 2014·Chemical Reviews·Qingjun LiuPing Wang
Jan 18, 2011·Biosensors & Bioelectronics·Julien FatissonNathalie Tufenkji

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