Novel reactions of L-rhamnose isomerase from Pseudomonas stutzeri and its relation with D-xylose isomerase via substrate specificity

Biochimica Et Biophysica Acta
Khim LeangKen Izumori

Abstract

Escherichia coli strain JM 109 harboring 6 x His-tag L-rhamnose isomerase (L-RhI) from Pseudomonas stutzeri allowed a 20-fold increase in the volumetric yield of soluble enzyme compared to the value for the intrinsic yield. Detailed studies on the substrate specificity of the purified His-tagged protein revealed that it catalyzed previously unknown common and rare aldo/ketotetrose, aldo/ketopentose, and aldo/ketohexose substrates in both D- and L-forms, for instance, erythrose, threose, xylose, lyxose, ribose, glucose, mannose, galactose, altrose, tagatose, sorbose, psicose, and fructose. Using a high enzyme-substrate ratio in extended reactions, the enzyme-catalyzed interconversion reactions from which two different products from one substrate were formed: L-lyxose, L-glucose, L-tagatose and D-allose were isomerized to L-xylulose and L-xylose, L-fructose and L-mannose, L-galactose and L-talose, and D-psicose and D-altrose, in that order. Kinetic studies, however, showed that L-rhamnose with Km and Vmax values of 11 mM and 240 U/mg, respectively, was the most preferred substrate, followed by L-mannose, L-lyxose, D-ribose, and D-allose. Based on the observed catalytic mode of action, these new findings reflected a hitherto undet...Continue Reading

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Citations

Jan 12, 2007·Applied Microbiology and Biotechnology·Tom Birger GranströmMatti Leisola
Jan 23, 2009·Applied Microbiology and Biotechnology·Ran-Young YoonDeok-Kun Oh
Sep 21, 2010·Applied Microbiology and Biotechnology·Ponnandy PrabhuJung-Kul Lee
Jun 10, 2011·Applied Microbiology and Biotechnology·Yu-Ri Lim, Deok-Kun Oh
Feb 22, 2012·Journal of Industrial Microbiology & Biotechnology·Koen BeerensWim Soetaert
Jun 7, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hiromi YoshidaShigehiro Kamitori
Nov 20, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Junho JungLin Woo Kang
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Jun 3, 2015·Journal of Bioscience and Bioengineering·Sirinan ShompoosangKenji Morimoto
Nov 6, 2008·Journal of Biotechnology·Ran-Young YoonDeok-Kun Oh
May 24, 2006·Journal of Biotechnology·Ken Izumori
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Oct 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tan-Viet PhamLin-Woo Kang
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Sep 9, 2010·Journal of Agricultural and Food Chemistry·Chia-Jui LinTsuei-Yun Fang
Aug 31, 2021·Biochemical and Biophysical Research Communications·Takayuki KuroishikawaYasuhiko Asada

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