Novel, real-time cell analysis for measuring viral cytopathogenesis and the efficacy of neutralizing antibodies to the 2009 influenza A (H1N1) virus.

PloS One
Di TianYunwen Hu

Abstract

A novel electronic cell sensor array technology, the real-time cell analysis (RTCA) system, was developed to monitor cell events. Unlike the conventional methods labeling the target cells with fluorescence, luminescence, or light absorption, the RTCA system allows for label-free detection of cell processes directly without the incorporation of labels. Here, we used this new format to measure the cytopathic effect (CPE) of the 2009 influenza A (H1N1) virus and the efficacy of neutralizing antibodies in human sera to this virus. The real-time dynamic monitoring of CPE was performed on MDCK cell cultures infected with the H1N1 virus, ranging from 5.50×10(2) to 5.50×10(7) copies/mL. The resulting CPE kinetic curves were automatically recorded and were both time and viral load dependent. The CPE kinetics were also distinguishable between different H1N1 stains, as the onset of CPE induced by the A/Shanghai/37T/2009 H1N1 virus was earlier than that of the A/Shanghai/143T/2009 H1N1 virus. Furthermore, inhibition of H1N1 virus-induced CPE in the presence of human specific anti-sera was detected and quantified using the RTCA system. Antibody titers determined using this new neutralization test correlated well with those obtained independ...Continue Reading

References

Jan 1, 1997·In Vitro Cellular & Developmental Biology. Animal·E NissenD Vollenbroich
Sep 11, 2004·Assay and Drug Development Technologies·Kelli SollyWei Zheng
Feb 22, 2005·Chemical Research in Toxicology·James Zan XingXiao Xu
Nov 23, 2006·Assay and Drug Development Technologies·Josephine M AtienzaYama A Abassi
Oct 27, 2007·BMJ : British Medical Journal·Vivienne NathansonGeorge Roycroft
Apr 17, 2008·Biotechnology Journal·Biao XiYama A Abassi
May 2, 2009·Journal of Virological Methods·Liisa LehtorantaThedi Ziegler
Sep 12, 2009·The New England Journal of Medicine·Kathy HancockJacqueline M Katz
Sep 17, 2009·Biological Procedures Online·Andrea StaršíchováKarel Souček
Oct 23, 2009·The New England Journal of Medicine·Feng-Cai ZhuJing-Shan Zheng
Nov 20, 2009·Environmental Science & Technology·Dorothy Yu HuangDavid Kinniburgh
Dec 17, 2010·The New England Journal of Medicine·Jiang WuYing Deng
Feb 26, 2011·Journal of Virological Methods·Ying FangWilliam Reisen
Nov 5, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chao QiuJianqing Xu

❮ Previous
Next ❯

Citations

Mar 22, 2014·Expert Review of Molecular Diagnostics·Bin HuangYi-Wei Tang
Apr 12, 2015·Journal of Laboratory Automation·Christopher F Fronczek, Jeong-Yeol Yoon
Mar 23, 2017·Journal of Microbiological Methods·Jiufeng SunDe Wu
May 1, 2015·Journal of Virology·Suhad Al-YahyaKhalid S A Khabar
Jan 15, 2015·Molecular and Cellular Biology·Qian ZhangXishan Wang
Sep 27, 2018·PloS One·Jennifer M ReimanChris Pierret
Aug 5, 2020·Nature Biomedical Engineering·Pavlo GilchukRobert H Carnahan
Nov 12, 2019·Analytical Chemistry·Vanessa VelascoRahim Esfandyarpour
Apr 9, 2018·Journal of Virological Methods·Galina BurmakinaAlexander Malogolovkin

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensor
PCR

Software Mentioned

RTCA
SPSS
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.