Novel recombinant feline interferon carrying N-glycans with reduced allergy risk produced by a transgenic silkworm system

BMC Veterinary Research
Sachi MinagawaSatoshi Sekiguchi

Abstract

The generation of recombinant proteins for commercialisation must be cost-effective. Despite the cost-effective production of recombinant feline interferon (rFeIFN) by a baculovirus expression system, this rFeIFN carries insect-type N-glycans, with core α 1,3 fucosyl residues that act as potential allergens. An alternative method of production may yield recombinant glycoproteins with reduced antigenicity. A cDNA clone encoding the fifteenth subtype of FeIFN-α (FeIFN-α15) was isolated from a Japanese domestic cat. This clone encoded a protein of 189 amino acids with a molecular mass of 21.1 kDa. The rFeIFN-α15 was expressed using a transgenic silkworm system, which was expected to yield an N-glycan structure with reduced antigenicity compared with the protein produced by the baculovirus system. The resulting rFeIFN-α15 accumulated in the sericin layer of silk fibres and was easily extracted and purified by column chromatography. The N-terminal amino acid sequence of purified rFeIFN-α15 was identical to the mature form of natural sequence. Moreover, its N-glycans did not include detectable core α 1,3 fucosyl residues. Its anti-vesicular stomatitis virus activity (2.6 × 108 units/mg protein) was comparable to that of the baculovir...Continue Reading

References

Oct 1, 1992·Trends in Biotechnology·M J Geisow
Feb 1, 1992·Bioscience, Biotechnology, and Biochemistry·N NakamuraA Yanai
Jun 1, 1992·The Journal of Veterinary Medical Science·T SakuraiA Yanai
May 1, 1985·Journal of Virology·K IatrouH Witkiewicz
Jun 1, 1985·Endocrinology·M S DordalE Goldwasser
Jan 1, 1981·Methods in Enzymology·P C FamillettiS Pestka
Oct 9, 1999·Veterinary Microbiology·T MinagawaT Kajimoto
Sep 5, 2002·Veterinary Immunology and Immunopathology·Ramani WonderlingMichael Milhausen
Dec 17, 2002·Nature Biotechnology·Masahiro TomitaKatsutoshi Yoshizato
Nov 19, 2003·Biochemical and Biophysical Research Communications·Ryo MisakiTatsuji Seki
Jul 9, 2004·The Journal of Veterinary Medical Science·Ayuko NagaiNobutoshi Maehara
Jun 9, 2005·The Journal of Veterinary Medical Science·Osamu TairaNobutoshi Maehara
Feb 24, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Li-Min YangWen-Jun Liu
Apr 7, 2007·Transgenic Research·Masahiro TomitaKatsutoshi Yoshizato
Nov 6, 2007·Current Drug Targets·Xianzong Shi, Donald L Jarvis
Sep 11, 2009·The FEBS Journal·Masashi IizukaMasahiro Tomita

❮ Previous
Next ❯

Citations

May 6, 2020·Transgenic Research·Sachi MinagawaMasahiro Tomita
Aug 10, 2020·Protein Expression and Purification·Hirofumi NakayaShigeki Takeda

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
glycosylation
column chromatography
gel filtration
protein assay

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.