Novel redox species polyaniline derivative-Au/Pt as sensing platform for label-free electrochemical immunoassay of carbohydrate antigen 199

Analytica Chimica Acta
Liyuan WangZhanfang Ma

Abstract

A novel electrochemical redox-active nanocomposite was synthesized by a one-pot method using N,N'-diphenyl-p-phenylediamine as monomer, and HAuCl4 and K2PtCl4 as co-oxidizing agents. The as-prepared poly(N,N'-diphenyl-p-phenylediamine)-Au/Pt exhibited admirable electrochemical redox activity at 0.15 V, excellent H2O2 electrocatalytic ability and favorable electron transfer ability. Based on these, the evaluation of the composite as sensing substrate for label-free electrochemical immunosensing to the sensitive detection of carbohydrate antigen 199 was described. This technique proved to be a prospective detection tool with a wide liner range from 0.001 U mL(-1) to 40 U mL(-1), and a low detection limit of 2.3 × 10(-4) U mL(-1) (S/N = 3). In addition, this method was used for the analysis of human serum sample, and good agreement was obtained between the values and those of enzyme-linked immunosorbent assay, implying the potential application in clinical research. Importantly, the strategy of the present substrate could be extended to other polymer-based nanocomposites such as polypyrrole derivatives or polythiophene derivatives, and this could be of great significance for the electrochemical immunoassay.

References

Nov 14, 2006·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·K S Goonetilleke, A K Siriwardena
Dec 18, 2007·American Journal of Surgery·Bulent C YukselSuleyman Hengirmen
May 26, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·P J MonaghanR Sodi
Oct 22, 2010·Biosensors & Bioelectronics·Baoxiang GuMingliang Wang
Dec 24, 2010·Biosensors & Bioelectronics·Wentao Shi, Zhanfang Ma
Jul 29, 2011·Chemical Society Reviews·Maëlle PerfézouArben Merkoçi
Jul 28, 2012·ACS Nano·Bhaskara V ChikkaveeraiahXiaoyuan Chen
Jun 2, 2015·Expert Review of Molecular Diagnostics·Zhanfang Ma, Na Liu
Jun 10, 2015·Biosensors & Bioelectronics·Saurabh KumarBansi D Malhotra
Jun 19, 2015·Scientific Reports·Nguyen Minh VuongHyojin Kim

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Citations

May 4, 2018·Expert Review of Molecular Diagnostics·Shuang Yin, Zhanfang Ma
Mar 6, 2021·Analytical and Bioanalytical Chemistry·Wenxian ZhangChonghai Xu

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