Novel resistance to imipenem associated with an altered PBP-4 in a Pseudomonas aeruginosa clinical isolate

The Journal of Antimicrobial Chemotherapy
F BellidoJ C Pechère

Abstract

A Pseudomonas aeruginosa (isolate 416) from a patient with pneumonia, was initially susceptible to imipenem (MIC: 2 mg/l) but became resistant to this antibiotic (isolate 470, MIC: 32 mg/l) during imipenem therapy. Treatment failed. No parallel increases in MIC were observed for other antimicrobials tested. Isolates 416 and 470 shared the same pyocin type and serotype, produced small amounts of an inducible beta-lactamase, and had similar lipopolysaccharide compositions. On electrophoresis of outer membrane proteins, the porin F, identified by the monoclonal antibody MA4-4, was expressed similarly by the two isolates but the production of one band (apparent molecular weight: 47,000) was diminished in isolate 470. [14C]-Imipenem labelling of intact cells proceeded more slowly in 470 than in 416, especially when bacterial cells were treated by antibody MA4-4 to block the porin F channel. [14C]-Imipenem labelling of penicillin binding proteins (PBP) showed that the band identified as PBP-4 bound markedly less radioactivity in isolate 470 than in 416. After isolate 470 was passaged several times in antibiotic-free broth, the imipenem MIC was decreased from 32 to 8 mg/l, and the [14C]-imipenem PBP pattern recovered the initial profi...Continue Reading

Citations

Nov 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C DibV Jarlier
Sep 1, 1992·International Journal of Antimicrobial Agents·A C Fluit, M R Visser
Feb 28, 1991·The New England Journal of Medicine·G A Jacoby, G L Archer
Aug 24, 2011·Antimicrobial Agents and Chemotherapy·Krisztina M Papp-WallaceRobert A Bonomo
Feb 1, 2012·Antimicrobial Agents and Chemotherapy·Alain A Ocampo-SosaUNKNOWN Spanish Network for Research in Infectious Diseases (REIPI)
Jan 1, 1991·Antimicrobial Agents and Chemotherapy·F BellidoR E Hancock
Mar 1, 1991·Antimicrobial Agents and Chemotherapy·M Michea-HamzehpourJ C Pechere
Apr 30, 1999·Mayo Clinic Proceedings·W C Hellinger, N S Brewer
Feb 6, 2008·FEMS Microbiology Reviews·André ZapunThierry Vernet
Mar 1, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Jean-Claude Pechère, Thilo Köhler
Jul 13, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C G GiskeG Kronvall
Sep 11, 2019·Current Infectious Disease Reports·Stephanie HoConan MacDougall
Oct 1, 1991·Antimicrobial Agents and Chemotherapy·M Michéa-HamzehpourJ C Pechère

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