Novel role for JNK as a stress-activated Bcl2 kinase

The Journal of Biological Chemistry
X DengW S May

Abstract

Interleukin (IL)-3-induced Bcl2 phosphorylation at Ser(70) may be required for its full and potent antiapoptotic activity. However, in the absence of IL-3, increased expression of Bcl2 can also prolong cell survival. To determine how Bcl2 may be functionally phosphorylated following IL-3 withdrawal, a stress-activated Bcl2 kinase (SAK) was sought. Results indicate that anisomycin, a potent activator of the stress kinase JNK/SAPK, can induce Bcl2 phosphorylation at Ser(70) and that JNK1 can be latently activated following IL-3 withdrawal to mediate Bcl2 phosphorylation. JNK1 directly phosphorylates Bcl2 in vitro, co-localizes with Bcl2, and collaborates with Bcl-2 to mediate prolonged cell survival in the absence of IL-3 or following various stress applications. Dominant-negative (DN)-JNK1 can block both anisomycin and latent IL-3 withdrawal-induced Bcl2 phosphorylation (>90%) and potently enhances cell death. Furthermore, low dose okadaic acid (OA), a potent protein phosphatase 1 and 2A inhibitor, can activate the mitogen-activated protein kinases JNK1 and ERK1/2, but not p38 kinase, to induce Bcl2 phosphorylation and prolong cell survival in factor-deprived cells. Since PD98059, a specific MEK inhibitor, can only partially inh...Continue Reading

References

Nov 24, 1995·Science·Z XiaM E Greenberg
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S HaldarC M Croce
May 12, 1994·Nature·J M KyriakisJ R Woodgett
Feb 2, 1996·The Journal of Biological Chemistry·C Y Chen, D V Faller
Nov 15, 1996·Cell·T F Gajewski, C B Thompson
Jan 23, 1997·Nature·A J MinnC B Thompson
May 2, 1997·The Journal of Biological Chemistry·T ItoW S May
May 9, 1998·Journal of Molecular and Cellular Cardiology·T L YueG Z Feuerstein
Apr 30, 1998·Current Opinion in Cell Biology·Y T Ip, R J Davis
Aug 28, 1998·Science·D R Green, J C Reed
Sep 12, 1998·Science·J M Adams, S Cory
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·R K SrivastavaD L Longo
Jan 5, 2000·The Journal of Biological Chemistry·S KharbandaD Kufe
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·X DengW S May

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Citations

Jun 22, 2006·Apoptosis : an International Journal on Programmed Cell Death·Kerstin BellmannJacques Landry
Dec 29, 2005·Journal of Clinical Immunology·Jun-Li LuoMichael Karin
Jan 21, 2004·Journal of Photochemistry and Photobiology. B, Biology·Sung-E ChoiYup Kang
Sep 11, 2002·Archives of Biochemistry and Biophysics·Nobuo WatanabeHenry Jay Forman
Apr 25, 2003·Biochemical and Biophysical Research Communications·Young-Mi HamSeung-Ki Lee
Oct 14, 2003·Biochemical Pharmacology·Nan-Shan ChangUdo Oppermann
Oct 14, 2003·Biochemical Pharmacology·Jacques PiettePatrizia Agostinis
May 23, 2002·Biochimie·Hans Friberg, Tadeusz Wieloch
May 3, 2003·Trends in Molecular Medicine·Peter M Kang, Seigo Izumo
Dec 13, 2005·Cell Death and Differentiation·H NakanoK Okumura
May 15, 2007·Oncogene·A J Whitmarsh, R J Davis
Mar 7, 2002·The Biochemical Journal·Christopher J DoughertyKeith A Webster
Apr 5, 2002·The Biochemical Journal·Xiao-Chun BaiShen-Qiu Luo
Jun 5, 2008·Antioxidants & Redox Signaling·Dunyaporn TrachoothamPeng Huang
Mar 28, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandra V AndreevaTatyana A Voyno-Yasenetskaya
May 13, 2010·The Journal of Pharmacology and Experimental Therapeutics·Shizu OyamadaFrank W Sellke
Sep 16, 2009·Infection and Immunity·Moo-Seung LeeVernon L Tesh
May 12, 2007·Journal of Virology·Arnaud AutretBruno Blondel
Jun 2, 2006·Molecular and Cellular Biology·Xingming DengW Stratford May
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Marie A Bogoyevitch, Bostjan Kobe
May 2, 2007·Gut·Harmeet MalhiGregory J Gores
Apr 17, 2013·Journal of Hematology & Oncology·Akintunde AkinleyeDelong Liu
May 10, 2002·Circulation Journal : Official Journal of the Japanese Circulation Society·Peter M KangSeigo Izumo
Mar 10, 2010·Future Microbiology·Vernon L Tesh
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Melanie A Simpson, Vinata B Lokeshwar
Nov 29, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Lei ZhouLanata Serggio
Feb 25, 2005·Neurosurgical Focus·Bimal G RamiSatyendra K Singh

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