Novel role for K+-dependent Na+/Ca2+ exchangers in regulation of cytoplasmic free Ca2+ and contractility in arterial smooth muscle

American Journal of Physiology. Heart and Circulatory Physiology
Hui DongJonathan Lytton

Abstract

Cytoplasmic free Ca2+ ([Ca2+]cyt) is essential for the contraction and relaxation of blood vessels. The role of plasma membrane Na+/Ca2+ exchange (NCX) activity in the regulation of vascular Ca2+ homeostasis was previously ascribed to the NCX1 protein. However, recent studies suggest that a relatively newly discovered K+-dependent Na+/Ca2+ exchanger, NCKX (gene family SLC24), is also present in vascular smooth muscle. The purpose of the present study was to identify the expression and function of NCKX in arteries. mRNA encoding NCKX3 and NCKX4 was demonstrated by RT-PCR and Northern blot in both rat mesenteric and aortic smooth muscle. NCXK3 and NCKX4 proteins were also demonstrated by immunoblot and immunofluorescence. After voltage-gated Ca2+ channels, store-operated Ca2+ channels, and Na+ pump were pharmacologically blocked, when the extracellular Na+ was replaced with Li+ (0 Na+) to induce reverse mode (Ca2+ entry) activity of Na+/Ca2+ exchangers, a large increase in [Ca2+]cyt signal was observed in primary cultured aortic smooth muscle cells. About one-half of this [Ca2+]cyt signal depended on the extracellular K+. In addition, after the activity of NCX was inhibited by KB-R7943, Na+ replacement-induced Ca2+ entry was abso...Continue Reading

References

Jan 1, 1991·Annals of the New York Academy of Sciences·M A KhoyiD P Westfall
Oct 1, 1991·The American Journal of Physiology·M A KhoyiD P Westfall
Aug 1, 1990·The American Journal of Physiology·S BovaM P Blaustein
Jan 1, 1987·Journal of Cardiovascular Pharmacology·T Ashida, M P Blaustein
Mar 15, 1973·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H ReuterG Haeusler
Jul 1, 1972·Microvascular Research·H BaumgärtlH Huland
Nov 1, 1995·The American Journal of Physiology·M K SlodzinskiM P Blaustein
Mar 1, 1995·Trends in Pharmacological Sciences·C van BreemenI Laher
Nov 1, 1993·The American Journal of Physiology·M A KhoyiD P Westfall
Jan 1, 1994·The American Journal of Physiology·M JuhaszovaM P Blaustein
Oct 4, 1996·The Journal of Biological Chemistry·D A NicollK D Philipson
Feb 1, 1997·British Journal of Pharmacology·H DongC R Triggle
Feb 12, 1998·The American Journal of Physiology·M A Nazer, C Van Breemen
Mar 28, 1998·The Journal of Biological Chemistry·M TsoiJ Lytton
Jul 3, 1999·Physiological Reviews·M P Blaustein, W J Lederer
Jan 25, 2000·American Journal of Physiology. Cell Physiology·A ArnonM P Blaustein
Mar 14, 2001·American Journal of Physiology. Cell Physiology·K D WuJ Lytton
May 23, 2001·Circulation Research·M Shigekawa, T Iwamoto
Sep 29, 2001·Trends in Neurosciences·M P Blaustein, V A Golovina
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Cheng-Han LeeCornelis van Breemen
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Cheng-Han LeeCornelis van Breemen
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yi-Hsien Su, Victor D Vacquier
Jul 27, 2002·Circulation Research·Hannes ReuterKenneth D Philipson
Oct 16, 2002·The Journal of Biological Chemistry·Xiao-Fang LiJonathan Lytton
Dec 28, 2002·Annals of the New York Academy of Sciences·Jonathan LyttonAlexander Kraev
Nov 20, 2003·The Journal of Biological Chemistry·Xinjiang Cai, Jonathan Lytton
Jan 23, 2004·The Journal of Biological Chemistry·Christian RoskerKlaus Groschner
Apr 3, 2004·The Journal of Biological Chemistry·Raz PaltyIsrael Sekler
Jun 1, 2004·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Nobuhiko TakaiYuji Imaizumi

❮ Previous
Next ❯

Citations

Dec 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiao DongHui Dong
Jun 24, 2011·American Journal of Physiology. Renal Physiology·Di ZhaoL Gabriel Navar
Jan 1, 2014·Genesis : the Journal of Genetics and Development·Vishal Sharma, Damien M O'Halloran
Dec 7, 2010·Progress in Neuro-psychopharmacology & Biological Psychiatry·Eric MickStephen V Faraone
Jun 21, 2016·Nature Genetics·Padhraig GormleyAarno Palotie
Sep 12, 2016·Current Hypertension Reports·Rossella IatrinoLaura Zagato
Aug 4, 2007·Progress in Neurobiology·Tamás L Török
Jun 13, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ting GuiYasuteru Muragaki
Mar 17, 2010·The Journal of Biological Chemistry·Haider F AltimimiPaul P M Schnetkamp
Apr 24, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Paola Algara-SuárezRicardo Espinosa-Tanguma
Jun 15, 2007·Physiology·Frank Visser, Jonathan Lytton
May 29, 2009·American Journal of Physiology. Renal Physiology·Geun-Shik LeeEui-Bae Jeung
Oct 20, 2010·European Journal of Clinical Investigation·Richárd HalmaiIstván Wittmann
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Aug 19, 2020·The FEBS Journal·Mattia D PizzagalliGiulio Superti-Furga
Jun 29, 2017·Human Genetics·Claudia PisanuHelgi B Schiöth
Nov 29, 2017·American Journal of Hypertension·Kuo LiuLing Zhang
Aug 25, 2007·The Biochemical Journal·Jonathan Lytton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.