Novel role of extracellular matrix protein 1 (ECM1) in cardiac aging and myocardial infarction

PloS One
Sean A HardyAndrew J Boyle

Abstract

The prevalence of heart failure increases in the aging population and following myocardial infarction (MI), yet the extracellular matrix (ECM) remodeling underpinning the development of aging- and MI-associated cardiac fibrosis remains poorly understood. A link between inflammation and fibrosis in the heart has long been appreciated, but has mechanistically remained undefined. We investigated the expression of a novel protein, extracellular matrix protein 1 (ECM1) in the aging and infarcted heart. Young adult (3-month old) and aging (18-month old) C57BL/6 mice were assessed. Young mice were subjected to left anterior descending artery-ligation to induce MI, or transverse aortic constriction (TAC) surgery to induce pressure-overload cardiomyopathy. Left ventricle (LV) tissue was collected early and late post-MI/TAC. Bone marrow cells (BMCs) were isolated from young healthy mice, and subject to flow cytometry. Human cardiac fibroblast (CFb), myocyte, and coronary artery endothelial & smooth muscle cell lines were cultured; human CFbs were treated with recombinant ECM1. Primary mouse CFbs were cultured and treated with recombinant angiotensin-II or TGF-β1. Immunoblotting, qPCR and mRNA fluorescent in-situ hybridization (mRNA-FISH)...Continue Reading

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Citations

Jun 9, 2020·Frontiers in Medicine·Kenneth P HoughVictor J Thannickal
Mar 18, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Shuaibo HuangNikolaos G Frangogiannis

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Methods Mentioned

BETA
coronary artery bypass
fluorescence microscopy
Assay
flow cytometry

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