Novel role of the nutraceutical bioactive compound berberine in lectin-like OxLDL receptor 1-mediated endothelial dysfunction in comparison to lovastatin

Nutrition, Metabolism, and Cardiovascular Diseases : NMCD
C CalicetiA F G Cicero

Abstract

Oxidized LDL (oxLDL) or pro-inflammatory stimuli lead to increased oxidative stress linked to endothelial dysfunction and atherosclerosis. The oxLDL receptor-1 (LOX1) is elevated within atheromas and cholesterol-lowering statins inhibit LOX1 expression. Berberine (BBR), an alkaloid extracted from plants of gender Berberis, has lipid-lowering and anti-inflammatory activity. However, its role in regulating LOX1-mediated signaling is still unknown. The aim of this study was to investigate the effect of BBR on oxLDL- and TNFα-induced endothelial dysfunction in human umbilical vein endothelial cells (HUVECs) and to compare it with that of lovastatin (LOVA). Cytotoxicity was determined by lactate dehydrogenase assay. Antioxidant capacity was measured with chemiluminescent and fluorescent method and intracellular ROS levels through a fluorescent dye. Gene and protein expression levels were assayed by qRT-PCR and western blot, respectively. HUVECs exposure to oxLDL (30 μg/ml) or TNFα (10 ng/ml) for 24 h led to a significant increase in LOX1 expression, effect abrogated by BBR (5 μM) and LOVA (5 μM). BBR but not LOVA treatment abolished the TNFα-induced cytotoxicity and restored the activation of Akt signaling. In spite of a low direct ...Continue Reading

Citations

Feb 12, 2019·Critical Reviews in Food Science and Nutrition·Shuzheng ChengMing Du
Aug 9, 2017·Annals of Medicine·Cesare R SirtoriMassimiliano Ruscica
May 29, 2018·Current Cardiology Reports·Vanessa BianconiMatteo Pirro
Jan 10, 2020·Oxidative Medicine and Cellular Longevity·Giorgio AquilaPaola Rizzo
Apr 27, 2020·Frontiers of Medicine·Danyang SongDaiming Fan
Jan 3, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Cai-Lan LiZi-Ren Su

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