Novel roles of ascorbate in plants: induction of cytosolic Ca2+ signals and efflux from cells via anion channels

Journal of Experimental Botany
M MakavitskayaV Demidchik

Abstract

Ascorbate is not often considered as a signalling molecule in plants. This study demonstrates that, in Arabidopsis roots, exogenous l-ascorbic acid triggers a transient increase of the cytosolic free calcium activity ([Ca2+]cyt.) that is central to plant signalling. Exogenous copper and iron stimulate the ascorbate-induced [Ca2+]cyt. elevation, while cation channel blockers, free radical scavengers, low extracellular [Ca2+], transition metal chelators, and removal of the cell wall inhibit this reaction. These data show that apoplastic redox-active transition metals are involved in the ascorbate-induced [Ca2+]cyt. elevation. Exogenous ascorbate also induces a moderate increase in programmed cell death symptoms in intact roots, but it does not activate Ca2+ influx currents in patch-clamped root protoplasts. Intriguingly, the replacement of gluconate with ascorbate in the patch-clamp pipette reveals a large ascorbate efflux current, which shows sensitivity to the anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. EPR spectroscopy measurements demonstrate that salinity (NaCl) triggers the accumulation of root apoplastic ascorbyl radicals in an A9C-dependent manner, con...Continue Reading

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Citations

Apr 25, 2018·International Journal of Molecular Sciences·Vadim Demidchik
Jun 23, 2018·Journal of Experimental Botany·Ismail Turkan
Oct 1, 2019·Plant Signaling & Behavior·Mansi Gogna, Satish C Bhatla
Mar 23, 2018·International Journal of Molecular Sciences·Igor PottosinSergey Shabala
Feb 11, 2020·Frontiers in Plant Science·Peilei ChenDorothea Bartels
Apr 29, 2019·Functional Plant Biology : FPB·Neha SinghVadim Demidchik
Sep 26, 2019·Frontiers in Plant Science·Limin WangJulia M Davies
May 16, 2019·International Journal of Molecular Sciences·Julia ShumilinaAndrej Frolov
Jun 23, 2018·Journal of Experimental Botany·Igor Pottosin, Isaac Zepeda-Jazo
Feb 5, 2021·Frontiers in Plant Science·Palina HryvusevichVadim Demidchik
May 28, 2019·Plant Science : an International Journal of Experimental Plant Biology·Matthew J MarcecKiwamu Tanaka
May 21, 2021·Current Opinion in Plant Biology·Regina GratzTzvetina Brumbarova

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