PMID: 9435461Jan 22, 1998Paper

Novel screen methodologies for identification of new microbial metabolites with pharmacological activity

Advances in Biochemical Engineering/biotechnology
D C HillL J Nisbet

Abstract

Micro-organisms continue to provide an important source of chemical diversity for the discovery of compounds with new biological activities. Microbial metabolites discovered recently using assays to detect compounds with potential pharmacological utility are surveyed and found to represent an extensive range of structural types produced by a wide variety of organisms. Assays used for screening samples produced by microbial processes must be robust, sensitive and specific and able to operate above a background of potential interferences from a number of sources. Discovery assays currently in use fall into three main categories cell-based, receptor-ligand interaction and enzyme inhibition assays. Trends in the use of these assays and new developments in assay technology applicable to the screening of microbial samples are examined with particular reference to the high throughput screening environment. For microbial screening to be a competitive route to new drug leads, the disciplines involved must be engineered into a seamlessly integrated process to deliver novel compounds with the required biological properties rapidly.

Citations

Mar 1, 2009·Current Protocols in Pharmacology·Michael EntzerothPeter Condron
Jan 9, 2009·Biomedical Microdevices·Zeta Tak For YuHsian-Rong Tseng
Jun 11, 1999·Analytical Biochemistry·M A Model, K E Healy
Feb 19, 2000·Current Opinion in Biotechnology·S A Sundberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.