Novel Segment- and Host-Specific Patterns of Enteroaggregative Escherichia coli Adherence to Human Intestinal Enteroids

MBio
Anubama RajanAnthony W Maresso

Abstract

Enteroaggregative Escherichia coli (EAEC) is an important diarrheal pathogen and a cause of both acute and chronic diarrhea. It is a common cause of pediatric bacterial diarrhea in developing countries. Despite its discovery in 1987, the intestinal tropism of the pathogen remains unknown. Cell lines used to study EAEC adherence include the HEp-2, T-84, and Caco-2 lines, but they exhibit abnormal metabolism and large variations in gene expression. Animal models either do not faithfully manifest human clinical symptoms or are cumbersome and expensive. Using human intestinal enteroids derived from all four segments of the human intestine, we find that EAEC demonstrates aggregative adherence to duodenal and ileal enteroids, with donor-driven differences driving a sheet-like and layered pattern. This contrasts with the colon, where segment-specific tropisms yielded a mesh-like adherence pattern dominated by interconnecting filaments. Very little to no aggregative adherence to jejunal enteroids was observed, regardless of the strain or donor, in contrast to a strong duodenal association across all donors and strains. These unique patterns of intestinal segment- or donor-specific adherence, but not the overall numbers of associated ba...Continue Reading

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Citations

Aug 1, 2018·Current Opinion in Infectious Diseases·Aldo A M LimaAlexandre Havt
Jan 16, 2019·Infection and Immunity·Benjamin J KoestlerShelley M Payne
Jan 16, 2019·Infection and Immunity·Sridevi RanganathanEileen M Barry
Oct 8, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sarah E BluttMary K Estes
Feb 28, 2020·Experimental & Molecular Medicine·Sungjin MinSeung-Woo Cho
Nov 7, 2019·Advances in Nutrition·Sara C Di Rienzi, Robert A Britton
Apr 18, 2019·Development·Emily M HollowayJason R Spence
Aug 19, 2020·Nature Materials·Masayuki Fujii, Toshiro Sato
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Feb 6, 2021·Biochemical Society Transactions·Chrysanthi-Maria Moysidou, Róisín M Owens
Jan 8, 2021·Annals of Biomedical Engineering·Reid L WilsonK Jane Grande-Allen
Mar 9, 2021·Frontiers in Pediatrics·Lauren O'ConnellCarol M Aherne
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Laura-Isobel McCall
Apr 16, 2018·Current Opinion in Virology·Sasirekha RamaniMary K Estes
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Nov 21, 2021·Nature Communications·Ana Teresa López-Jiménez, Serge Mostowy

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Methods Mentioned

BETA
biopsy
PCR
genotyping

Software Mentioned

GraphPad Prism
Graph Pad

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