PMID: 11927978Apr 3, 2002Paper

Novel serum replacement based on bovine ocular fluid: a useful tool for cultivation of different animal cells in vitro

ALTEX
Bratko FilipicSrecko Koren

Abstract

Different mammalian cells in culture have individual nutritional requirements, which are mainly fulfilled by the addition of foetal calf serum (FCS) to the basic medium. Collecting FCS is accompanied with severe animal welfare problems as conscient animals usually are bleeded to death by heart-punctuation without anaesthetics. There exists scientific problems too. Due to the batch-to-batch variability and the relatively high price, different types of serum replacements were introduced. Among them bovine colostrum as a serum substitute for the cultivation of hybridoma cells should be mentioned. The presented experiments were aimed to introduce the simple and effective serum replacement (SR) based on the bovine ocular fluid. Throughout the experiments the bovine ocular fluid alone and in the combination with the sheep's defibrinated plasma and human serum albumin was tested for the growth of different cells growing as a monolayer: (a) Cell lines: WISH (human amniotic cell lines) and VERO. (b) Primary culture: chicken embryonic fibroblasts, human bone-marrow fibroblasts. All growth experiments were performed in parallel with the Foetal Calf Serum (FCS) of three different sources. All types of cells were cultivated in Eagle's mediu...Continue Reading

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