Novel Set-Up for Low-Disturbance Sampling of Volatile and Non-volatile Compounds from Plant Roots

Journal of Chemical Ecology
Elisabeth J EilersAndreas Reinecke

Abstract

Most studies on rhizosphere chemicals are carried out in substrate-free set-ups or in artificial substrates using sampling methods that require an air flow and may thus cause disturbance to the rhizosphere. Our study aimed to develop a simplified and inexpensive system that allows analysis of rhizosphere chemicals at experimentally less disturbed conditions. We designed a mesocosm in which volatile rhizosphere chemicals were sampled passively (by diffusion) without air- and water flow on polydimethylsiloxane-(PDMS) tubes. Dandelion (Taraxacum sect. ruderalia) was used as model plant; roots were left undamaged. Fifteen volatiles were retrieved from the sorptive material by thermal desorption for analysis by gas chromatography/mass spectrometry (GC/MS). Furthermore, three sugars were collected from the rhizosphere substrate by aqueous extraction and derivatized prior to GC/MS analysis. In order to study how the quantity of detected rhizosphere compounds depends on the type of soil or substrate, we determined the matrix-dependent recovery of synthetic rhizosphere chemicals. Furthermore, we compared sorption of volatiles on PDMS tubes with and without direct contact to the substrate. The results show that the newly designed mesocos...Continue Reading

References

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Citations

Feb 3, 2016·Trends in Plant Science·Nicole M van Dam, Harro J Bouwmeester
Dec 19, 2018·Frontiers in Plant Science·Friederike Carolin CreyaufmüllerBarbara Vornam
Jan 25, 2021·Analytica Chimica Acta·Cecilia CaglieroPatrizia Rubiolo
Dec 28, 2018·The Plant Pathology Journal·Rouhallah Sharifi, Choong-Min Ryu
Jan 29, 2021·The Plant Journal : for Cell and Molecular Biology·Dorothea ThollQingshan Wei

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Methods Mentioned

BETA
ion-exchange chromatography

Software Mentioned

R
R Development Core Team
MassFinder

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