Novel spectroscopic technique for in situ monitoring of collagen fibril alignment in gels

Biophysical Journal
Oksana Kostyuk, Robert A Brown

Abstract

Development of collagen fibril alignment in contracting fibroblast-populated and externally tensioned acellular collagen gels was studied using elastic scattering spectroscopy. Spectra of the backscattered light (320-860 nm) were acquired with a 2.75-mm source-detector separation probe placed perpendicular to the gel surface and rotated to achieve different angles to the collagen fibril alignment. Backscatter was isotropic for noncontracted/unloaded gels (disorganized matrix). As gels were contracted/externally loaded (collagen alignment developed), anisotropy of backscatter increased: more backscatter was detected perpendicular than parallel to the direction of the fibril alignment. An "anisotropy factor" (AF) was calculated to characterize this effect as the ratio of backscatter intensities at orthogonal positions. Before contraction (or zero strain) the AF was close to unity at all wavelengths. In contrast, at 72 h, backscatter anisotropy varied from AF(400 nm) = 2.14 +/- 0.29 to AF(700 nm) = 3.04 +/- 0.48. It also increased over threefold up to a strain of 20%. The AF strongly correlated with the contraction time/strain. Different directions of the backscatter were detected in gel zones with known differences in the matrix ...Continue Reading

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Citations

Mar 24, 2007·Annals of Biomedical Engineering·Aron Parekh, Darrell Velegol
May 6, 2008·Tissue Engineering. Part B, Reviews·Alvin T YehAdam M Larson
Oct 11, 2008·Tissue Engineering. Part B, Reviews·Irene GeorgakoudiDavid L Kaplan
Oct 12, 2010·Journal of the Royal Society, Interface·Alvena KureshiRobert Brown
Oct 27, 2005·Journal of Biomechanical Engineering·Stavros ThomopoulosJeffrey W Holmes
Mar 27, 2009·Lasers in Medical Science·Mario A TrellesMarwan Al-Zarouni
Jul 31, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Spencer P Lake, Victor H Barocas
Feb 19, 2009·Dermatologic Therapy·Claudia van der LugtMario A Trelles
Jan 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Aron ParekhPatricia A Hebda
Nov 19, 2011·Journal of Clinical Periodontology·Sahng G KimHyun-Duck Nah
Jun 24, 2016·Journal of the Royal Society, Interface·Marios GeorgiadisPhilipp Schneider
May 23, 2006·Journal of Biomedical Materials Research. Part a·D KaramichosV Mudera

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