Novel splicing isoforms of synaptotagmin-like proteins 2 and 3: identification of the Slp homology domain

Biochemical and Biophysical Research Communications
M FukudaK Mikoshiba

Abstract

Slp1-3 (synaptotagmin-like protein 1-3) is a new family of carboxyl-terminal-type (C-type) tandem C2 proteins that show higher sequence similarity to the C2 domains of granuphilin-a/Slp-4 than those of other C-type tandem C2 proteins (e.g., synaptotagmin and the Doc2 family). However, the amino (N)-terminal domains of the original Slp1-3 do not contain any known protein motifs and do not show any sequence similarities to each other. We report four alternative splicing isoforms of Slp2 (designated Slp2-a-d, with the original Slp2 renamed Slp2-c) and two alternative splicing isoforms of Slp3 (Slp3-a and Slp3-b, the original Slp3). These isoforms share the same C-terminal tandem C2 structures, but their N-terminal nucleotide sequences are completely different due to the alternate use of different exons. Sequence alignment of the Slp1, Slp2-a, Slp3-a, and Slp4 amino terminal domains reveals the presence of two conserved regions among the Slp family, designated SHD1 (Slp homology domain 1) and SHD2, which may function as protein interaction sites. The SHD1 and SHD2 of Slp3-a and Slp4 are separated by a putative Zn(2+)-binding sequence, whereas Slp1 and Slp2 lack such Zn(2+)-binding sequences and their SHD1 and SHD2 are linked togeth...Continue Reading

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Citations

Jul 24, 2012·Nature Cell Biology·Manuel Gálvez-SantistebanFernando Martín-Belmonte
Nov 22, 2001·The Biochemical Journal·M Fukuda, K Mikoshiba
Sep 11, 2007·The Journal of Biological Chemistry·April S GoehringJohn D Scott
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Oct 1, 2003·Molecular Biology of the Cell·Laurent WaselleRomano Regazzi
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Jul 16, 2003·Molecular and Cellular Biology·Taruho S KurodaMitsunori Fukuda
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Jun 6, 2003·Biochemical and Biophysical Research Communications·Mitsunori Fukuda
Mar 24, 2006·Biochemical and Biophysical Research Communications·Mitsunori Fukuda
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Jun 15, 2019·International Journal of Molecular Medicine·Qiang ZhuJianyong Gao

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