Abstract
Altered expressions of receptor tyrosine kinases drive the growth and metastasis of several cancers. RON is a single pass transmembrane receptor tyrosine kinase (RTK) shown to be aberrantly expressed in various cancer types. However, target validation and successful therapeutic targeting of RON in cancers is hampered by the co-existence of unknown number/types of isoforms, which are structurally similar but functionally diverse. The objective of this study was to identify differential splicing in the C-terminal region of RON transcripts to better understand RON signaling in cancers. mRNA transcript sequence between exons 14 and 20 of RON was PCR amplified and sequenced using cDNA from 10 SCLC and 13 NSCLC cell lines. Specific exon deletions were identified by aligning sequencing chromatograms with reference RON cDNA sequence. We identified the presence of four unique transcript sequence variants of RON formed through skipping of exons 15-19, 16-19, 16-17 and 16. The transcript variants, except the one lacking exons 15-19, were found in more than one cell line. Several cell lines contained two to four of these uniquely spliced transcript variants. dbEST (Expressed Sequence Tags database) or other DNA sequence databases did not c...Continue Reading
References
Jul 10, 1996·Experimental Cell Research·M H WangE J Leonard
Feb 15, 1997·The Journal of Clinical Investigation·O SakamotoT Suda
Jul 22, 1998·Oncogene·P MaggioraP M Comoglio
Jul 27, 2000·Carcinogenesis·M H WangY Chen
Oct 11, 2001·Oncogene·B E PeaceS E Waltz
Sep 6, 2002·Oncogene·Yi-Qing ChenMing-Hai Wang
Jan 16, 2003·Oncogene·Yong-Qing ZhouMing-Hai Wang
Jun 17, 2003·Carcinogenesis·Ming-Hai WangYi-Qing Chen
Sep 21, 2004·Oncogene·Xiang-Ming XuMing-Hai Wang
Feb 14, 2007·Oncogene·D AngeloniM I Lerman
Feb 22, 2007·Cancer·E Ramsay CampLee M Ellis
May 26, 2007·Briefings in Functional Genomics & Proteomics·Debora Angeloni
Jul 6, 2007·Cancer Biology & Therapy·Ming-Hai WangHang-Ping Yao
Jul 10, 2007·Cancer Research·Ryan M ThomasAndrew M Lowy
Sep 25, 2007·Cancer Letters·Yi LuMing-Hai Wang
Jul 3, 2008·Arthritis Research & Therapy·Pei JinEwa M Paleolog
Dec 2, 2008·BMC Cancer·Donghui ZhouXiaodong Teng
Feb 20, 2009·The Journal of Biological Chemistry·Jing WangMichael G Brattain
Jun 13, 2009·Journal of Neurochemistry·Carmen EckerichKatrin Lamszus
Jan 28, 2010·Cancer Research·Jocelyn Logan-CollinsAndrew M Lowy
Apr 30, 2010·International Journal of Oncology·Qi MaMing-Hai Wang
Feb 3, 2011·Translational Oncology·Zeynep GunesAlessandra Vitelli
May 16, 2012·Genes, Chromosomes & Cancer·Rajani KantetiRavi Salgia
Oct 3, 2013·Cold Spring Harbor Perspectives in Biology·Graham Carpenter, Hong-Jun Liao
Jun 7, 2014·Cancer Research·Hong-Yi ChangNan-Haw Chow
Citations
Nov 5, 2016·Journal of Cancer Research and Clinical Oncology·Omid ZareiSiavoush Dastmalchi
Feb 18, 2017·International Journal of Molecular Sciences·Cherine Abou-FayçalBeatrice Eymin
Nov 11, 2017·BMC Cancer·Soundararajan KrishnaswamyNasser M Al-Daghri
Dec 19, 2018·Veterinary Pathology·Lorella ManiscalcoRaffaella De Maria
May 8, 2018·Oncotarget·Valeriy PoroykoRavi Salgia
Aug 10, 2017·Oncology Letters·Zulei LiHui Tian
Jul 4, 2019·Molecular Cancer Research : MCR·Jacqueline OlenderNorman H Lee