Novel splicing variants of recepteur d'origine nantais (RON) tyrosine kinase involving exons 15-19 in lung cancer

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Soundararajan KrishnaswamyNasser M Al-Daghri

Abstract

Altered expressions of receptor tyrosine kinases drive the growth and metastasis of several cancers. RON is a single pass transmembrane receptor tyrosine kinase (RTK) shown to be aberrantly expressed in various cancer types. However, target validation and successful therapeutic targeting of RON in cancers is hampered by the co-existence of unknown number/types of isoforms, which are structurally similar but functionally diverse. The objective of this study was to identify differential splicing in the C-terminal region of RON transcripts to better understand RON signaling in cancers. mRNA transcript sequence between exons 14 and 20 of RON was PCR amplified and sequenced using cDNA from 10 SCLC and 13 NSCLC cell lines. Specific exon deletions were identified by aligning sequencing chromatograms with reference RON cDNA sequence. We identified the presence of four unique transcript sequence variants of RON formed through skipping of exons 15-19, 16-19, 16-17 and 16. The transcript variants, except the one lacking exons 15-19, were found in more than one cell line. Several cell lines contained two to four of these uniquely spliced transcript variants. dbEST (Expressed Sequence Tags database) or other DNA sequence databases did not c...Continue Reading

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Citations

Nov 5, 2016·Journal of Cancer Research and Clinical Oncology·Omid ZareiSiavoush Dastmalchi
Feb 18, 2017·International Journal of Molecular Sciences·Cherine Abou-FayçalBeatrice Eymin
Dec 19, 2018·Veterinary Pathology·Lorella ManiscalcoRaffaella De Maria

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