PMID: 9524202Apr 3, 1998Paper

Novel structural difference between nopaline- and octopine-type trbJ genes: construction of genetic and physical map and sequencing of trb/traI and rep gene clusters of a new Ti plasmid pTi-SAKURA

Biochimica Et Biophysica Acta
K SuzukiK Yoshida

Abstract

We constructed a gene library of a nopaline-type Ti plasmid (called pTi-SAKURA) which was newly isolated from Agrobacterium tumefaciens MAFF301001 of a Japanese cherry tree. The partial sequencing data, which were distributed over the entire plasmid genome, made it possible to assign typical Ti-encoded genes including trb and rep gene clusters. The trb/traI and rep gene clusters were sequenced completely. All the genes in the regions except trbJ were homologous with the corresponding genes on octopine-type Ti plasmids, based on both ORF size and sequence similarity. The trbJ on pTi-SAKURA is similar to that of an octopine-type Ti, but has an extra 282-base segment in its central domain. The above gene organization and sequences suggest a divergence of Ti plasmid during evolution in relation to Rhizobium plasmids, and is discussed in this paper.

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Citations

Mar 9, 2004·Plasmid·Nathalie CorneauGisèle LaPointe
May 16, 2000·Journal of Bacteriology·M A Ramírez-RomeroM A Cevallos
Jun 27, 2000·Journal of Bacteriology·J ZhuS C Winans
Apr 25, 2008·Plasmid·Miguel A CevallosRosa María Gutiérrez-Ríos
Jul 12, 2005·Plasmid·Javier IzquierdoMiguel A Cevallos
Jul 31, 2007·Genes & Genetic Systems·Shinji YamamotoKatsunori Suzuki

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