Novel substrates of a ribose-5-phosphate isomerase from Clostridium thermocellum

Journal of Biotechnology
Ran-Young YoonDeok-Kun Oh

Abstract

A substrate specificity study of the recombinant D-ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum was performed. Among all aldopentoses and aldohexoses, the RpiB enzyme displayed activity with L-talose, D-ribose, D-allose, L-allose, L-ribose, and D-talose in decreasing order. The products released were L-tagatose, D-ribulose, D-psicose, L-psicose, L-ribulose, and D-tagatose, respectively. The enzyme showed specificity for aldose substrates possessing hydroxyl groups oriented in the same direction at the C2, C3, and C4 positions. Molecular modeling of the enzyme suggests that the novel substrate specificity may be explained by substrate interactions with residues Tyr42, His98, and His9, which interact with the hydroxyl groups of C2, C3, and C4, respectively, oriented in the same direction. L-Talose and D-ribulose exhibited the highest activity among the aldoses and ketoses, respectively. Ribose 5-phosphate isomerase catalyzed the conversion of L-talose to L-tagatose with an 89% conversion yield after approximately 90 min, while D-ribulose was converted to D-ribose with a 38% conversion yield.

References

Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Dec 1, 1996·Glycobiology·S MuniruzzamanA D Elbein
May 25, 2002·Journal of Nutritional Science and Vitaminology·Tatsuhiro MatsuoKen Izumori
Jan 4, 2003·Journal of Medicinal Food·Gilbert V Levin
Dec 23, 2003·Journal of Molecular Biology·Annette K RoosSherry L Mowbray
Jul 6, 2004·Journal of Hepato-biliary-pancreatic Surgery·Mohammad Akram HossainHajime Maeta
Oct 20, 2005·Journal of Bioscience and Bioengineering·Akiko MurataMasaaki Tokuda

Citations

Jan 23, 2009·Applied Microbiology and Biotechnology·Ran-Young YoonDeok-Kun Oh
Dec 7, 2010·Applied Microbiology and Biotechnology·Soo-Jin YeomDeok-Kun Oh
Jun 10, 2011·Applied Microbiology and Biotechnology·Yu-Ri Lim, Deok-Kun Oh
Nov 20, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Junho JungLin-Woo Kang
Jun 15, 2010·Journal of Bioscience and Bioengineering·Hyun-Jung KwonDeok-Kun Oh
Jul 8, 2015·Applied Microbiology and Biotechnology·Wanmeng MuBo Jiang
Oct 24, 2014·PLoS Computational Biology·Seth D AxenCheryl A Kerfeld
Jun 28, 2016·Applied Microbiology and Biotechnology·Ziwei ChenWanmeng Mu
Feb 29, 2016·Applied Biochemistry and Biotechnology·Manisha J PatelDarshan H Patel
Jan 9, 2015·PLoS Neglected Tropical Diseases·Inês LoureiroJoana Tavares
Dec 25, 2013·Beilstein Journal of Organic Chemistry·Zijie LiLi Cai
Jul 4, 2018·Applied Microbiology and Biotechnology·Zheng FangWanmeng Mu
Jun 14, 2020·Applied Microbiology and Biotechnology·Jiajun ChenWanmeng Mu
Jun 26, 2020·The Journal of Microbiology·Soo-Jung KimSoo-Jin Yeom
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nikola MijailovicAziz Aziz
Jun 12, 2021·Enzyme and Microbial Technology·Rong WangLiangzhi Li

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