Novel Thermostable Flavin-binding Fluorescent Proteins from Thermophilic Organisms

Photochemistry and Photobiology
Marcus WingenThomas Drepper

Abstract

Flavin-binding fluorescent proteins (FbFPs) are small, oxygen-independent in vivo reporters, derived from Light Oxygen Voltage (LOV) domains of photoreceptors. Here, we investigated the thermostability of existing, as well as novel FbFPs, whose genes were identified in genome sequences of various thermophilic bacteria as well as metagenomic libraries from hot springs in the Yellowstone National Park. Detailed in vitro analyses revealed that two of those fluorescent reporter proteins were highly thermostable, exhibiting melting temperatures above 75°C.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Nov 1, 1995·Trends in Biochemical Sciences·A B CubittR Y Tsien
Oct 18, 2001·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·P A van den BergA J Visser
Apr 20, 2002·Biophysical Journal·Aba LosiWolfgang Gärtner
Sep 21, 2002·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yasukazu NakamuraSatoshi Tabata
Jun 2, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Aba Losi
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Dec 22, 2005·Nature Biotechnology·Jean-Denis PédelacqGeoffrey S Waldo
Aug 31, 2006·Extremophiles : Life Under Extreme Conditions·Karl O Stetter
Oct 27, 2006·Current Opinion in Structural Biology·S James Remington
Feb 21, 2007·Accounts of Chemical Research·Rebekka M Wachter
Mar 14, 2007·Nature Biotechnology·Thomas DrepperKarl-Erich Jaeger
Sep 4, 2007·Journal of Molecular Biology·Andreas Möglich, Keith Moffat
Apr 2, 2008·Annals of the New York Academy of Sciences·Isaac D Wagner, Juergen Wiegel
Dec 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sean ChapmanJohn M Christie
Mar 17, 2009·Systematic and Applied Microbiology·Angel AngelovWolfgang Liebl
Apr 15, 2009·Protein Engineering, Design & Selection : PEDS·Csaba KissAndrew R M Bradbury
Jul 29, 2010·Physiological Reviews·Dmitriy M ChudakovKonstantin A Lukyanov
Nov 16, 2010·Applied and Environmental Microbiology·William T BeesonMichael A Marletta
Feb 10, 2011·Standards in Genomic Sciences·Brian J TindallAlla Lapidus
Nov 3, 2011·Photochemistry and Photobiology·Gopal P PathakWolfgang Gärtner
Mar 21, 2012·Molecular Plant·John M ChristieAndrew J Roe
May 11, 2012·The Journal of Biological Chemistry·John M ChristieElizabeth D Getzoff
May 9, 2013·Advances in Applied Microbiology·Benedikt LeisWolfgang Liebl
May 11, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thomas DrepperMartina Pohl
Oct 11, 2013·Methods in Molecular Biology·Klaus SuhlingPei-Hua Chung
Oct 30, 2013·The International Journal of Developmental Biology·Ute VossDarren M Wells
Feb 7, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Marcus WingenThomas Gensch
May 3, 2014·Applied Microbiology and Biotechnology·Kristen BlanchardIchiro Matsumura
Nov 6, 2014·Life·Francesco Canganella, Juergen Wiegel
Apr 29, 2015·Biotechnology Advances·M Sofía UrbietaKian Mau Goh
May 25, 2015·Advances in Applied Microbiology·Ali H HusseinDavid J Leak
May 30, 2015·Current Opinion in Chemical Biology·Alexander S MishinKonstantin A Lukyanov

❮ Previous
Next ❯

Citations

May 23, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Vera V NazarenkoIvan Gushchin
Oct 12, 2018·Scientific Reports·Stephan EndresThomas Drepper
Oct 19, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Eleonora ConsiglieriAba Losi
May 12, 2018·Microorganisms·Eivind B DrejerTrygve Brautaset
Jun 22, 2021·Biochemical and Biophysical Research Communications·Ivan M GoncharovIvan Gushchin
Dec 4, 2019·Bioconjugate Chemistry·Harun F OzbakirArnab Mukherjee
Jul 10, 2018·Chemical Reviews·Aba LosiAndreas Möglich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.