Novel thymine-functionalized polystyrenes for applications in biotechnology. 2. Adsorption of model proteins

Biomacromolecules
Judit E PuskasMichael Cunningham

Abstract

This paper investigates the adsorption of bovine serum albumin (BSA) and bovine hemoglobin (BHb) model proteins onto novel thymine-functionalized polystyrene (PS-VBT) microspheres, in comparison with polystyrene (PS) microspheres. Maximum adsorption was obtained for both proteins near their corresponding isoelectric points (pI at pH = 4.7 for BSA and 7.1 for BHb). FTIR and adsorption isotherm analysis demonstrated that, although both proteins were physisorbed onto PS through nonspecific hydrophobic interactions, adsorption onto the functionalized copolymers occurred by both physisorption and chemisorption via hydrogen bonding. FTIR analysis also indicated conformational changes in the secondary structure of BSA and BHb adsorbed onto PS, whereas little or no conformation change was seen in the case of adsorption onto PS-VBT. Atomic force microscopy (AFM), consistent with the isotherm results, also demonstrated monolayer adsorption for both proteins. AFM images of BSA adsorbed onto copolymers with 20 mol % surface VBT loading showed exclusively end-on orientation. Adsorption onto copolymers with lower functionality showed mixed end-on and side-on orientation modes of BSA, and only the side-on orientation was observed on PS. The A...Continue Reading

References

Feb 1, 1987·Journal of Biomedical Materials Research·D R AbsolomA W Neumann

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Citations

Oct 19, 2007·Bioprocess and Biosystems Engineering·Yaser Dahman, Argyrios Margaritis
Jan 1, 2009·Biomicrofluidics·Walter SchrottDalimil Snita
Jul 10, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Priscilla JohnstonKei Saito
Oct 23, 2012·Journal of Colloid and Interface Science·E QuirogaA J Ramirez-Pastor
Jul 7, 2009·Colloids and Surfaces. B, Biointerfaces·Cecília F G FerreiraPaulo C Camargo
Mar 18, 2008·Colloids and Surfaces. B, Biointerfaces·Qiang MaXing-Guang Su
Aug 30, 2014·Journal of the Mechanical Behavior of Biomedical Materials·A EscudeiroA Cavaleiro
Nov 17, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Anne ValléeClaire-Marie Pradier

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