Novel triple‑positive markers identified in human non‑small cell lung cancer cell line with chemotherapy-resistant and putative cancer stem cell characteristics

Oncology Reports
Nazilah Abdul SatarPuteri Baharuddin

Abstract

Through the specific identification and direct targeting of cancer stem cells (CSCs), it is believed that a better treatment efficacy of cancer may be achieved. Hence, the present study aimed to identify a CSC subpopulation from adenocarcinoma cells (A549) as a model of non‑small cell lung cancer (NSCLC). Ιnitially, we sorted two subpopulations known as the triple‑positive (EpCAM+/CD166+/CD44+) and triple‑negative (EpCAM-/CD166-/CD44-) subpopulation using fluorescence-activated cell sorting (FACS). Sorted cells were subsequently evaluated for proliferation and chemotherapy-resistance using a viability assay and were further characterized for their clonal heterogeneity, self-renewal characteristics, cellular migration, alkaline dehydrogenase (ALDH) activity and the expression of stemness-related genes. According to our findings the triple‑positive subpopulation revealed significantly higher (P<0.01) proliferation activity, exhibited better clonogenicity, was mostly comprised of holoclones and had markedly bigger (P<0.001) spheroid formation indicating a better self-renewal capacity. A relatively higher resistance to both 5‑fluouracil and cisplatin with 80% expression of ALDH was observed in the triple‑positive subpopulation, com...Continue Reading

Citations

Feb 23, 2019·Scientific Reports·Maria José CostaShu-Hui Liu
Aug 28, 2020·Frontiers in Immunology·Lia WalcherUta Kossatz-Boehlert
Feb 23, 2021·Oncotarget·Beatrice AraminiKhawaja Husnain Haider

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Methods Mentioned

BETA
Fluorescence
flow cytometry
PCR
xenografts

Software Mentioned

SPSS
BD [UNK] Pro

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