Novel triterpene oxidizing activity of Arabidopsis thaliana CYP716A subfamily enzymes

FEBS Letters
Shuhei YasumotoToshiya Muranaka

Abstract

Triterpenoids have diverse chemical structures and bioactivities. Cytochrome P450 monooxygenases play a key role in their structural diversification. In higher plants, CYP716A subfamily enzymes are triterpene oxidases. In this study, Arabidopsis thaliana CYP716A1 and CYP716A2 were characterized by heterologously expressing them in simple triterpene-producing yeast strains. In contrast to the C-28 oxidative activity of CYP716A1 shown in several CYP716A subfamily enzymes, remarkably, CYP716A2 displayed 22α-hydroxylation activity against α-amyrin that has not been previously reported, which produces the cytotoxic triterpenoid, 22α-hydroxy-α-amyrin. Our results contribute to the enrichment of the molecular toolbox that allows for the combinatorial biosynthesis of diverse triterpenoids. This article is protected by copyright. All rights reserved.

References

May 1, 1995·Plant Physiology·M. A. JenksK. A. Feldmann
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hikaru SekiToshiya Muranaka
Apr 29, 2009·PloS One·Franziska B MullauerJan Paul Medema
May 19, 2009·Organic Letters·Pietro MorlacchiSeiichi P T Matsuda
Dec 3, 2009·Human Genomics·David R Nelson
May 25, 2010·Phytochemistry·Marianela SánchezJorge A Palermo
May 18, 2011·Natural Product Reports·Anne OsbournRobert A Field
Nov 1, 2011·Plant & Cell Physiology·Ery O FukushimaToshiya Muranaka
Feb 4, 2012·The Arabidopsis Book·Søren BakDanièle Werck-Reichhart
Jan 1, 2011·Frontiers in Plant Science·Satoru Sawai, Kazuki Saito
Jan 9, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Björn Hamberger, Søren Bak
Feb 5, 2013·Plant & Cell Physiology·Ery O FukushimaToshiya Muranaka
Apr 11, 2013·Journal of the American Chemical Society·Dorianne A CastilloSeiichi P T Matsuda
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tessa MosesAlain Goossens
Feb 7, 2014·Annual Review of Plant Biology·Ramesha ThimmappaAnne Osbourn
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alexander M BoutanaevAnne Osbourn
May 9, 2015·Plant & Cell Physiology·Hikaru SekiToshiya Muranaka

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Citations

Jul 28, 2016·Plant Science : an International Journal of Experimental Plant Biology·Jennifer Fiallos-JuradoAntonio Leon-Reyes
Mar 27, 2018·Plant & Cell Physiology·Ok Tae KimReinhard Jetter
Nov 25, 2017·Frontiers in Plant Science·Sumit Ghosh
Oct 21, 2018·The Plant Journal : for Cell and Molecular Biology·Yi Shang, Sanwen Huang
May 10, 2019·Critical Reviews in Biotechnology·Wentao SunChun Li
Mar 23, 2018·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Aparajita Banerjee, Björn Hamberger
Feb 15, 2017·Frontiers in Plant Science·Shuhei YasumotoToshiya Muranaka
Jul 15, 2021·ACS Synthetic Biology·Weixian LiZigang Qian
Nov 6, 2018·Journal of Agricultural and Food Chemistry·Yu-Jia Zhao, Chun Li

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