Novel two-parameter flow cytometry (MIL4/SSC followed by MIL4/CT7) allows for identification of five fractions of guinea pig leukocytes in peripheral blood and lymphoid organs

Journal of Immunological Methods
Mari TakizawaMitsuo Honda

Abstract

Though the guinea pig has been an extremely useful animal model for a variety of diseases, the tools necessary to undertake a full-scale immunological analysis of the guinea pig have been lacking. For instance, traditional two-parameter forward/side scatter (FSC/SSC) flow cytometry, though effective in human and other animal models, is unable to adequately identify the distinct fractions of guinea pig peripheral blood leukocytes (PBL). We introduce here a new flow cytometric technique (MIL4/SSC followed by MIL4/CT7) which redresses this lack by identifying and characterizing five distinct fractions of PBL: neutrophils, lymphocytes, monocytes, eosinophils plus basophils, and the novel MIL4(-)SSC(large)CT7(high) population. The MIL4(-)SSC(large)CT7(high) cells possess cytoplasmic inclusion bodies of variable size that were positive for periodic acid Schiff (PAS). Their cell surface stained positive for the helper/inducer lymphocyte markers, T cell markers, CD45, Thy-1, asialo GM1 and FcR, but negative for B cell markers, such as membrane-type IgM, CD8 and MHC class II. The novel flow cytometric technique also allowed us to establish that the five leukocyte fractions were found in PBL, splenocytes, thymocytes and lymph node cells....Continue Reading

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Citations

Feb 18, 2010·Antimicrobial Agents and Chemotherapy·Diane J OrdwayIan M Orme
Aug 4, 2010·International Journal of Radiation Biology·Ryuji OkazakiToshiyuki Norimura
May 9, 2008·Pulmonary Pharmacology & Therapeutics·Brendan J Canning, Yangling Chou
Feb 5, 2014·The American Journal of Pathology·Brendan K PodellRandall J Basaraba
Oct 26, 2018·Veterinary Clinical Pathology·Samantha J M EvansAmy L MacNeill
Apr 13, 2018·Journal of Virology·Christine A BricaultDan H Barouch
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diane OrdwayRandall J Basaraba

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