Novel type II and monomeric NAD+ specific isocitrate dehydrogenases: phylogenetic affinity, enzymatic characterization, and evolutionary implication

Scientific Reports
Peng WangGuoping Zhu

Abstract

NAD(+) use is an ancestral trait of isocitrate dehydrogenase (IDH), and the NADP(+) phenotype arose through evolution as an ancient adaptation event. However, no NAD(+)-specific IDHs have been found among type II IDHs and monomeric IDHs. In this study, novel type II homodimeric NAD-IDHs from Ostreococcus lucimarinus CCE9901 IDH (OlIDH) and Micromonas sp. RCC299 (MiIDH), and novel monomeric NAD-IDHs from Campylobacter sp. FOBRC14 IDH (CaIDH) and Campylobacter curvus (CcIDH) were reported for the first time. The homodimeric OlIDH and monomeric CaIDH were determined by size exclusion chromatography and MALDI-TOF/TOF mass spectrometry. All the four IDHs were demonstrated to be NAD(+)-specific, since OlIDH, MiIDH, CaIDH and CcIDH displayed 99-fold, 224-fold, 61-fold and 37-fold preferences for NAD(+) over NADP(+), respectively. The putative coenzyme discriminating amino acids (Asp326/Met327 in OlIDH, Leu584/Asp595 in CaIDH) were evaluated, and the coenzyme specificities of the two mutants, OlIDH R(326)H(327) and CaIDH H(584)R(595), were completely reversed from NAD(+) to NADP(+). The detailed biochemical properties, including optimal reaction pH and temperature, thermostability, and metal ion effects, of OlIDH and CaIDH were further...Continue Reading

References

Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·R ChenA M Dean
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Feb 1, 1997·Journal of Bacteriology·D G CvitkovitchA S Bleiweis
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·A M Dean, G B Golding
Sep 11, 1998·Science·J P Amend, E L Shock
Feb 24, 2001·Archives of Biochemistry and Biophysics·R Chen, H Yang
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sharmistha BanerjeeSeyed E Hasnain
Jan 4, 2005·Biochemistry. Biokhimii︠a︡·I G MorgunovT V Finogenova
Jan 18, 2005·Science·Guoping ZhuAntony M Dean
May 17, 2005·Nature Medicine·Ernesto J Muñoz-Elías, John D McKinney
Jun 25, 2005·The Journal of Biological Chemistry·Yu Chu Huang, Roberta F Colman
Apr 20, 2006·The Journal of Biological Chemistry·Gang HuLee McAlister-Henn
Jun 2, 2006·The Journal of Biological Chemistry·Sambanthamurthy SoundarRoberta F Colman
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Feb 8, 2008·The Journal of Biological Chemistry·Alexander B TaylorLee McAlister-Henn
Feb 16, 2008·Archives of Biochemistry and Biophysics·Qian LuLee McAlister-Henn
Aug 2, 2008·Biochimica Et Biophysica Acta·Feng QiDaniel A Beard
Sep 6, 2008·Science·D Williams ParsonsKenneth W Kinzler
Aug 7, 2009·The New England Journal of Medicine·Elaine R MardisTimothy J Ley
Nov 7, 2009·Journal of Pharmaceutical Sciences·Tsutomu ArakawaJohn S Philo
Nov 26, 2009·Nature·Lenny DangShinsan M Su

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Citations

Apr 7, 2020·The FEBS Journal·Shelley D Copley
Aug 28, 2020·PLoS Computational Biology·Saurav Mallik, Dan S Tawfik
Jun 24, 2017·Protein Expression and Purification·Xinying WangShigeharu Harada
Oct 15, 2021·Protein Science : a Publication of the Protein Society·Changqing TianGuoping Zhu

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Datasets Mentioned

BETA
ABP01147
EJP74315

Methods Mentioned

BETA
genetic modification
electrophoresis
protein assay
X-ray

Software Mentioned

BLAST
Clustal
Clustal X
TOF
MEGA
TOF Series Explorer
Prism

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