PMID: 357Dec 1, 1975

Novel type of murein transglycosylase in Escherichia coli

Journal of Bacteriology
J V HöltjeU Schwarz

Abstract

The purification and properties of a novel type of murein transglycosylase from Escherichia coli are described. The purified enzyme appears as a single band on sodium dodecyl sulfate-polyacrylamide gels and has an apparent molecular weight of approximately 65,000 as estimated by gel filtration and gel electrophoresis. It degrades pure murein sacculi from E. coli almost completely into low-molecular-weight products. The two prominent muropeptide fragments in the digest are the disaccharide-tripeptide N-acetylglucosamine-N-acetylmuramic acid-L-alanine-D-iso-glutamic acid-meso-diaminopimelic acid and the corresponding disaccharide-tetrapeptide N-acetylglucosamine-N-acetylmuramic acid-L-alanine-D-iso-glutamic acid-meso-diaminopimelic acid-D-alanine. The unique feature of these compounds is that the disaccharide has no reducing end group and that the muramic acid residue possesses an internal 1 leads to 6 anhydro linkage. The new lytic enzyme is designated as a murein: murein transglycosylase. Its possible role in the rearrangement of murein during cell growth and division is discussed.

Citations

May 1, 1995·Molecular Microbiology·K EhlertM F Templin
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·A J Dijkstra, W Keck
Sep 11, 2004·FEBS Letters·Christopher W ReidAnthony J Clarke
Mar 29, 2005·Antimicrobial Agents and Chemotherapy·Dorota KorsakWaldemar Vollmer
Sep 13, 2005·Protein Science : a Publication of the Protein Society·Jimin Pei, Nick V Grishin
Mar 21, 2006·Biochemistry. Biokhimii︠a︡·Konstantin A MiroshnikovVadim V Mesyanzhinov
Aug 7, 2007·Journal of Bacteriology·Melanie R StapletonSimon J Foster
Sep 18, 2007·Journal of Bacteriology·Javier de la MoraGeorges Dreyfus
Feb 13, 2008·FEMS Microbiology Reviews·Waldemar VollmerSimon J Foster
Mar 31, 2010·The Journal of Biological Chemistry·Chelsea A ClarkeAnthony J Clarke
Oct 12, 2010·The Biochemical Journal·Vincent LombardBernard Henrissat
Dec 12, 2012·Annals of the New York Academy of Sciences·Alexander J F Egan, Waldemar Vollmer
Mar 8, 1982·FEBS Letters·B SzewczykA Taylor
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·K G PayieAnthony J Clarke
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M von RechenbergJ V Höltje
May 15, 1981·European Journal of Biochemistry·E H BeacheyU Schwarz
Aug 1, 1993·FEMS Microbiology Letters·T RomeisJ V Höltje
Mar 9, 2000·Microbiology·T J SmithSimon J Foster
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·J ZupanP Zambryski
Apr 2, 2005·Protein Science : a Publication of the Protein Society·Jimin Pei, Nick V Grishin
Dec 28, 2007·The Journal of Biological Chemistry·Andrei FokineMichael G Rossmann
Feb 3, 2009·Journal of Bacteriology·Jared D HeffronDavid L Popham
Feb 24, 2012·Gut Microbes·Benoit MarteynPhilippe Sansonetti
Aug 7, 2012·Applied and Environmental Microbiology·Y S LaptevaI E Granovsky
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Jan 22, 2015·Critical Reviews in Biotechnology·Lorena Rodríguez-RubioPilar García
May 6, 2014·The Journal of Biological Chemistry·Patrick J Moynihan, Anthony J Clarke
Aug 10, 2000·Molecular Microbiology·Pia S Rydman, Dennis H Bamford
May 2, 1985·European Journal of Biochemistry·W KeckU Schwarz
Jan 22, 1998·Canadian Journal of Microbiology·S R Watt, Anthony J Clarke
Dec 14, 2001·Journal of Bacteriology·Pia S Rydman, Dennis H Bamford
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·James T Park, Tsuyoshi Uehara
Jun 24, 2008·Journal of Bacteriology·Karen A Cloud-HansenJoseph P Dillard
Oct 12, 2012·Canadian Journal of Microbiology·Hendrik StratingAnthony J Clarke
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Oleksii SliusarenkoThierry Emonet
Sep 25, 2014·The Journal of Biological Chemistry·Francesca A HerliheyAnthony J Clarke
Apr 8, 2015·The FEBS Journal·Vadim D NikitushkinArseny S Kaprelyants
Oct 4, 2012·Future Microbiology·Mathias SchmelcherMartin J Loessner
Sep 1, 2018·Canadian Journal of Microbiology·Anthony J Clarke
Dec 1, 1984·Archives of Microbiology·S Mollner, V Braun
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J V Höltje
Jan 1, 1981·Molecular & General Genetics : MGG·K Bienkowska-SzewczykA Taylor
Feb 8, 2005·Current Microbiology·Noriaki SakataToshiji Mukai
Aug 24, 2010·Journal of Bacteriology·Graham ChristieChristopher R Lowe
Dec 5, 2012·Infection and Immunity·David E PayneBlaise R Boles
Dec 12, 2007·FEMS Microbiology Reviews·Waldemar Vollmer
Oct 1, 1995·Archives of Microbiology·J V Höltje
Jan 11, 2014·Molecular Microbiology·Robert D TurnerSimon J Foster

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