Novel tyramide-based tyrosinase assay for the detection of melanoma cells in cytological preparations

Diagnostic Cytopathology
Cesar AngelettiDavid L Rimm

Abstract

Fine-needle aspiration (FNA) has been used as a fast, minimally invasive, and reliable method for the evaluation of enlarged lymph nodes. However, there are some cases where the definitive diagnosis cannot be elicited with morphology alone, especially cases without a known primary lesion. Although immunocytochemical studies may be helpful in some situations, they are often complicated by nonspecific staining. Recently, a novel tyramide-based tyrosinase assay was developed. Since melanocytes, both benign and malignant, produce tyrosinase, we postulated that this assay could be useful as an in situ biochemical diagnostic test. We modified the Perkin Elmer TSA assay, a commercial assay based on tyramide, a tyrosine analog that is a substrate for tyrosinase, for use on air-dried cytological preparations. We validated the assay on cell lines, then tested a small series of melanoma and nonmelanoma cytology specimens. The YUGEN8 melanoma cell line was used to optimize the assay and it showed abundant reaction product, while HeLa cells served as a negative control. All melanoma cytology specimens were positive and all nonmelanoma specimens were negative. These results suggest that this simple, fast, and inexpensive assay is a sensitive...Continue Reading

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Citations

Dec 22, 2006·The FEBS Journal·Itamar WillnerBilha Willner
Aug 10, 2007·Chemistry : a European Journal·Ronit FreemanItamar Willner
Oct 31, 2006·Biosensors & Bioelectronics·Itamar WillnerBilha Willner
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Oct 25, 2011·Chemical Communications : Chem Comm·Qingling Xu, Juyoung Yoon
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Apr 20, 2021·Methods and Applications in Fluorescence·Asanka Amith RajapakshaGuang-Fu Yang
Nov 30, 2006·Journal of the American Chemical Society·Ron GillUri Banin

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