Novel urinary exosomal biomarkers of acute T cell-mediated rejection in kidney transplant recipients: A cross-sectional study

PloS One
Jeong-Hoon LimChan-Duck Kim

Abstract

Acute rejection is hazardous to graft survival in kidney transplant recipients (KTRs). We aimed to identify novel biomarkers for early diagnosis of acute T cell-mediated rejection (TCMR) in urinary exosomes of KTRs. Among 458 graft biopsies enrolled in a cross-sectional multicenter study, 22 patients with stable graft function (STA) who had not shown pathologic abnormality and 25 patients who diagnosed biopsy-proven TCMR were analyzed. We performed proteomic analysis using nano-ultra performance liquid chromatography-tandem mass spectrometry (nano-UPLC-MS/MS) to identify candidate biomarkers for early TCMR diagnosis on urinary exosomes. We confirmed the protein levels of each candidate biomarker by western blot analysis. A total of 169 urinary exosome proteins were identified by nano-UPLC-MS/MS. Forty-six proteins showed increased expression in STA patients, while 17 proteins were increased in TCMR patients. Among them, we selected five proteins as candidate biomarkers for early diagnosis of TCMR according to significance, degree of quantity variance, and information from the ExoCarta database. We confirmed the proteomic expression levels of five candidate biomarkers by western blot analysis in each patient. Of all candidate bi...Continue Reading

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Citations

Jun 7, 2019·Journal of Zhejiang University. Science. B·Ai-Bin ZhangShu-Sen Zheng
Mar 17, 2020·Omics : a Journal of Integrative Biology·Shiva KalantariNooshin Dalili
Sep 24, 2020·International Journal of Molecular Sciences·Francesco GuzziPaola Romagnani
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Aug 6, 2020·International Journal of Molecular Sciences·Marco QuagliaVincenzo Cantaluppi
Nov 24, 2020·Hypertension·Olga Martinez-ArroyoRaquel Cortes
Mar 5, 2021·Journal of the American Society of Nephrology : JASN·Rania El FekihJamil R Azzi
Apr 20, 2021·Kidney Research and Clinical Practice·Sul A LeeTae-Hyun Yoo
Oct 19, 2019·Analytical Chemistry·Suttipong SuttapitugsakulRonghu Wu

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Methods Mentioned

BETA
biopsies
Protein Assay
biopsy
electrophoresis

Software Mentioned

MASCOT Distiller
MASCOT
Search Tool for the Retrieval of
SPSS

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