Novel vaccination approach for dengue infection based on recombinant immune complex universal platform

Vaccine
Mi-Young KimHugh S Mason

Abstract

Dengue infection is on the rise in many endemic areas of the tropics. Vaccination remains the most realistic strategy for prevention of this potentially fatal viral disease but there is currently no effective vaccine that could protect against all four known serotypes of the dengue virus. This study describes the generation and testing of a novel vaccination approach against dengue based on recombinant immune complexes (RIC). We modelled the dengue RIC on the existing Ebola RIC (Phoolcharoen, et al. Proc Natl Acad Sci USA 2011;108(Dec (51)):20695) but with a key modification that allowed formation of a universal RIC platform that can be easily adapted for use for other pathogens. This was achieved by retaining only the binding epitope of the 6D8 ant-Ebola mAb, which was then fused to the consensus dengue E3 domain (cEDIII), resulting in a hybrid dengue-Ebola RIC (DERIC). We expressed human and mouse versions of these molecules in tobacco plants using a geminivirus-based expression system. Following purification from the plant extracts by protein G affinity chromatography, DERIC bound to C1q component of complement, thus confirming functionality. Importantly, following immunization of mice, DERIC induced a potent, virus-neutrali...Continue Reading

References

Dec 20, 2002·Biotechnology and Bioengineering·Tsafrir S MorHugh S Mason
Aug 23, 2005·Infection and Immunity·Daniel ChargelegueJulian K-C Ma
Dec 31, 2008·Microbes and Infection·Chih-Hsiang LengHsin-Wei Chen
Nov 30, 2010·Vaccine·Charmagne G BeckettKevin R Porter
Jan 12, 2011·Annual Review of Immunology·Brian R Murphy, Stephen S Whitehead
Feb 2, 2011·Plant Biotechnology Journal·Waranyoo PhoolcharoenHugh S Mason
Apr 26, 2011·Current Opinion in Immunology·Beth-Ann G Coller, David E Clements
Jul 23, 2011·Vaccine·Janine R DankoKevin R Porter
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Waranyoo PhoolcharoenMelissa M Herbst-Kralovetz

❮ Previous
Next ❯

Citations

Jan 12, 2016·Human Vaccines & Immunotherapeutics·Hugh S Mason
Jan 19, 2016·Frontiers in Plant Science·Holger SpiegelRainer Fischer
Sep 22, 2015·Plant Biotechnology Journal·Stephen J StreatfieldVidadi Yusibov
Jun 16, 2015·Vaccine·Jorge A Salazar-GonzálezSergio Rosales-Mendoza
Oct 19, 2016·International Journal of Molecular Sciences·Young Hee JoungHyun-Soon Kim
Dec 10, 2017·Plant Biotechnology Journal·Mi Young KimRajko Reljic
Apr 24, 2018·Plant Biotechnology Journal·Gina R WebsterJulian K-C Ma
Aug 29, 2017·Human Vaccines & Immunotherapeutics·Edward Peter Rybicki
Feb 6, 2020·Frontiers in Bioengineering and Biotechnology·Andrew G DiamosHugh S Mason
Jan 25, 2019·NPJ Vaccines·Xuan-Yi WangYu-Mei Wen
May 19, 2017·Bioengineering & Translational Medicine·Timothy Z ChangJulie A Champion
Jul 1, 2020·Advanced Drug Delivery Reviews·Darrell J IrvineMurillo Silva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

© 2022 Meta ULC. All rights reserved