Novel Vector Construction Based on Alternative Adenovirus Types via Homologous Recombination

Human Gene Therapy Methods
Wenli ZhangAnja Ehrhardt

Abstract

Adenoviral vector (AdV) is one of the most used vectors in gene therapy clinical trials. However the therapeutic effect of AdV is limited due to preexisting immunity to the currently used human adenovirus type 5 and pre-decided vector tropism. It is highly demanded to develop novel AdVs originated from other types than adenovirus type 5. Here, we describe a method for direct cloning of adenovirus utilizing linear-linear homologous recombination, followed by rapid adenoviral genome modification via linear-circular homologous recombination. A plasmid bearing chosen adenoviral genome with the desired modification is generated in three weeks, from which a novel AdV can be reconstituted.

References

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Citations

May 7, 2020·Cancers·Jian GaoAnja Ehrhardt
Nov 30, 2018·Current Protocols in Mouse Biology·Yong Hong ChenBeverly L Davidson
Sep 6, 2020·International Journal of Molecular Sciences·Wenli ZhangAnja Ehrhardt
May 26, 2021·Human Vaccines & Immunotherapeutics·Mark VerdeciaFouad Atouf

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Methods Mentioned

BETA
genetic modification
transfection
PCR

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