Novel whole-tissue quantitative assay of nitric oxide levels in Drosophila neuroinflammatory response

Journal of Visualized Experiments : JoVE
Rami R Ajjuri, Janis M O'Donnell

Abstract

Neuroinflammation is a complex innate immune response vital to the healthy function of the central nervous system (CNS). Under normal conditions, an intricate network of inducers, detectors, and activators rapidly responds to neuron damage, infection or other immune infractions. This inflammation of immune cells is intimately associated with the pathology of neurodegenerative disorders, such as Parkinson's disease (PD), Alzheimer's disease and ALS. Under compromised disease states, chronic inflammation, intended to minimize neuron damage, may lead to an over-excitation of the immune cells, ultimately resulting in the exacerbation of disease progression. For example, loss of dopaminergic neurons in the midbrain, a hallmark of PD, is accelerated by the excessive activation of the inflammatory response. Though the cause of PD is largely unknown, exposure to environmental toxins has been implicated in the onset of sporadic cases. The herbicide paraquat, for example, has been shown to induce Parkinsonian-like pathology in several animal models, including Drosophila melanogaster. Here, we have used the conserved innate immune response in Drosophila to develop an assay capable of detecting varying levels of nitric oxide, a cell-signal...Continue Reading

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Citations

Sep 5, 2019·Scientific Reports·Urmila MaitraJanis M O'Donnell
Feb 22, 2018·Infection and Immunity·Upasana ShokalIoannis Eleftherianos
Jan 13, 2021·Chemosphere·Fernanda Neves EstrelaGuilherme Malafaia

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Methods Mentioned

BETA
dissection
Assay
dissections
electrophoresis
X-ray
chemical treatment
transgenic

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