Novel yeast killer toxins provoke S-phase arrest and DNA damage checkpoint activation

Molecular Microbiology
Roland KlassenFriedhelm Meinhardt

Abstract

Certain strains of Pichia acaciae and Wingea robertsiae (synonym Debaryomyces robertsiae) harbour extranuclear genetic elements that confer a killer phenotype to their host. Such killer plasmids (pPac1-2 of P. acaciae and pWR1A of W. robertsiae) were sequenced and compared with the zymocin encoding pGKL1 of Kluyveromyces lactis. Both new elements were found to be closely related to each other, but they are only partly similar to pGKL1. As for the latter, they encode functions mediating binding of the toxin to the target cell's chitin and a hydrophobic region potentially involved in uptake of a toxin subunit by target cells. Consistently, mutations affecting the target cell's major chitin synthase (Chs3) protect it from toxin action. Heterologous intracellular expression of respective open reading frames identified cell cycle-arresting toxin subunits deviating structurally from the likewise imported gamma-subunit of the K. lactis zymocin. Accordingly, toxicity of both P. acaciae and Wingea toxins was shown to be independent of RNA polymerase II Elongator, which is indispensable for zymocin action. Thus, P. acaciae and Wingea toxins differ in their mode of action from the G1-arresting zymocin. Fluorescence-activated cell sorting ...Continue Reading

References

Jul 1, 1991·Journal of General Microbiology·A R ButlerM J Stark
Oct 1, 1994·Current Genetics·N GungeF Meinhardt
Jul 1, 1997·Clinical Microbiology Reviews·W MaglianiL Polonelli
Aug 21, 2001·Molecular Cell·C S GilbertN F Lowndes
Aug 8, 2002·FEMS Microbiology Reviews·Manfred J Schmitt, Frank Breinig
Nov 26, 2002·Trends in Cell Biology·Alexander J OsbornLee Zou
Dec 28, 2002·Genes & Development·Kenji ShimadaSusan M Gasser
Sep 19, 2003·Molecular Genetics and Genomics : MGG·R Klassen, F Meinhardt

❮ Previous
Next ❯

Citations

Aug 18, 2012·Applied Microbiology and Biotechnology·Dhira SatwikaFriedhelm Meinhardt
Dec 29, 2010·Molecular Genetics and Genomics : MGG·Roland KlassenFriedhelm Meinhardt
May 8, 2007·Applied and Environmental Microbiology·John P PaluszynskiFriedhelm Meinhardt
Dec 5, 2006·Applied and Environmental Microbiology·Michele Di GiacomoDino Parente
Aug 12, 2009·Eukaryotic Cell·A Carolin Frank, Kenneth H Wolfe
Sep 27, 2013·PloS One·Megumi ShigematsuHaruhiko Masaki
Feb 24, 2007·Applied Microbiology and Biotechnology·Giovanni VigliottaPietro Alifano
Nov 10, 2012·Fungal Genetics and Biology : FG & B·Antonio SantosDomingo Marquina
Feb 1, 2005·Cellular Microbiology·Roland Klassen, Friedhelm Meinhardt
Jun 6, 2008·Molecular Microbiology·Roland KlassenFriedhelm Meinhardt
Dec 7, 2013·Molecular Microbiology·Alene KastFriedhelm Meinhardt
Mar 28, 2012·Biochemical and Biophysical Research Communications·Megumi ShigematsuHaruhiko Masaki
Oct 10, 2013·Critical Reviews in Biotechnology·Guang-Lei LiuZhen-Ming Chi
Sep 13, 2008·FEMS Microbiology Letters·Walter MaglianiLuciano Polonelli
Apr 25, 2006·FEMS Yeast Research·Roland KlassenFriedhelm Meinhardt
Jul 30, 2016·PloS One·Sabrina WemhoffFriedhelm Meinhardt
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Elaine G RodriguesLuiz R Travassos
Dec 20, 2016·Journal of Applied Microbiology·N N MehlomakuluB Divol
Aug 17, 2014·Applied and Environmental Microbiology·Sabrina WemhoffFriedhelm Meinhardt
Oct 3, 2019·World Journal of Microbiology & Biotechnology·Florian M FreimoserQuirico Migheli
Feb 3, 2005·Yeast
Dec 13, 2018·Frontiers in Genetics·Megumi ShigematsuYohei Kirino
Nov 4, 2020·Microorganisms·Mariana Andrea DíazJulián Rafael Dib

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.