Sep 1, 1976

Novikoff hepatoma deoxyribonucleic acid polymerase. Sensitivity of the beta-polymerase to sulfhydryl blocking agents

Nucleic Acids Research
D W MosbaughR R Meyer

Abstract

Unlike other beta-class eukaryotic DNA polymerases, the enzyme purified from the Novikoff hepatoma is inhibited by both sulfhydryl blocking agents N-ethylmaleimide (NEM) and p-hydroxymercuribenzoate (pHMB). The degree of sensitivity varies depending on the enzyme purity, pH of the reaction, and the presence of sulfhydryl reducing agents. Novikoff beta-polymerase activity is unaffected by the presence of 2-mercaptoethanol (2-Me) or dithiothreitol (DTT); however, the combination of 2-mercaptoethanol and NEM or pHMB acts to reverse the inhibition of the sulfhydryl blocking agent. The reversal of inhibition involves more than just a titration of NEM with 2-mercaptoethanol since a) the combination of these two reagents actually stimulates the DNA polymerase, and b) dithiothreitol did not reverse the inhibition. Binding of the polymerase to DNA did not affect the enzyme sensitivity to NEM.

  • References10
  • Citations8

References

  • References10
  • Citations8

Citations

Mentioned in this Paper

Ethylmaleimide
4-hydroxymercuribenzoate
Polymerase
Alkalescens-Dispar Group
2-Mercaptoethanol
Liver Carcinoma
Sputolysin
Dithiothreitol
Sulfhydryl Compounds
Hepatoma, Novikoff

About this Paper

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