NOX4-driven ROS formation regulates proliferation and apoptosis of gastric cancer cells through the GLI1 pathway

Cellular Signalling
Chao-Tao TangZhi-Zheng Ge

Abstract

NADPH Oxidase 4 (NOX4), a member of the NOX family, has emerged as a significant source of reactive oxygen species, playing an important role in tumor cell proliferation, apoptosis, and other physiological processes. However, the potential function of NOX4 in gastric cancer (GC) cell proliferation is yet unknown. The aim of this study was to illustrate whether NOX4 plays a role in regulating gastric cancer cell growth. First, the clinical information from 90 patients was utilized to explore the clinical value of NOX4 as a predictive tool for tumor size and prognosis. Results showed that NOX4 expression was correlated with tumor size and prognosis. In vitro assays confirmed that knockdown of NOX4 expression blocked cell proliferation and the expression of Cyclin D1, BAX, and so on. Interestingly, NOX4 promoted cell proliferation via activation of the GLI1 pathway. GLI1, a well-known transcription factor in the Hedgehog signaling pathway, was overexpressed to test whether NOX4 activates downstream signaling via GLI1. Overexpression of GLI1 reversed the inhibition of proliferation induced by NOX4 knockdown. In addition, overexpression of NOX4 increased GLI1 expression, and depletion of GLI1 expression decreased the effects induced...Continue Reading

Citations

Jul 31, 2018·Journal of Digestive Diseases·Chao Tao TangZhi Zheng Ge
Oct 20, 2018·Antioxidants & Redox Signaling·Rhian M TouyzAugusto C Montezano
Aug 29, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y LiX Xu
Aug 21, 2019·Frontiers in Cardiovascular Medicine·Denise BurtenshawPaul A Cahill
Aug 31, 2019·Oxidative Medicine and Cellular Longevity·Anastasiya V SnezhkinaAlexey A Dmitriev
Mar 27, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xinzhao WangZhiyong Yu

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