PMID: 10729120Mar 23, 2000Paper

NP and L proteins of lymphocytic choriomeningitis virus (LCMV) are sufficient for efficient transcription and replication of LCMV genomic RNA analogs

Journal of Virology
K J LeeJuan Carlos de la Torre


The genome of lymphocytic choriomeningitis virus (LCMV) consists of two negative-sense single-stranded RNA segments, designated L and S. Both segments contain two viral genes in an ambisense coding strategy, with the genes being separated by an intergenic region (IGR). We have developed a reverse genetic system that allows the investigation of cis-acting signals and trans-acting factors involved in transcription and replication of LCMV. To this end, we constructed an LCMV S minigenome consisting of a negative-sense copy of the chloramphenicol acetyltransferase (CAT) reporter gene flanked upstream by the S 5' untranslated region (UTR) and IGR and downstream by the S 3' UTR. CAT expression was detected in LCMV-infected cells transfected with the minigenome RNA. Intracellular coexpression of the LCMV minigenome and LCMV L and NP proteins supplied from cotransfected plasmids driven by the T7 RNA polymerase provided by the recombinant vaccinia virus vTF7-3 resulted in high levels of CAT activity and synthesis of subgenomic CAT mRNA and antiminigenome RNA species. Thus, L and NP represent the minimal viral trans-acting factors required for efficient RNA synthesis mediated by LCMV polymerase.


Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P L CollinsD S Stec
May 1, 1991·Virology·S IapalucciM T Franze-Fernández
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O Elroy-Stein, B Moss
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Apr 1, 1995·Trends in Microbiology·P Palese
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·N D LawsonJ K Rose
Jan 1, 1993·Annual Review of Microbiology·A García-Sastre, P Palese
Jan 1, 1994·Virology·P Borrow, M B Oldstone
Feb 1, 1994·Journal of Virology·K K Conzelmann, M Schnell
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·P L CollinsH Grosfeld
Mar 1, 1996·The Journal of General Virology·K K Conzelmann
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·J K Rose
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Bridgen, R M Elliott
Jun 11, 1998·Trends in Biotechnology·A García-Sastre

❮ Previous
Next ❯


Apr 5, 2013·Archives of Virology·Frank LennartzWolfgang Garten
Sep 27, 2002·Antiviral Research·C C GarcíaE B Damonte
Jun 5, 2003·Virus Research·Marie Nguyen, Anne-Lise Haenni
Apr 4, 2003·International Journal of Antimicrobial Agents·Cybele C GarcíaElsa B Damonte
Feb 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sebastien F EmonetJuan C de la Torre
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andreas BergthalerDaniel D Pinschewer
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Philip J Kranzusch, Sean P J Whelan
Dec 1, 2010·Antimicrobial Agents and Chemotherapy·Michelle MendenhallBrian B Gowen
Oct 29, 2010·Journal of Virology·Linda BrunotteStephan Günther
Jul 25, 2009·Journal of Virology·Sudhakar S AgnihothramJack H Nunberg
Sep 29, 2006·Journal of Virology·Meike HassStephan Günther
Aug 10, 2012·Journal of Virology·Nicholas L BairdJack H Nunberg
Nov 26, 2010·Journal of Virology·Giulia PasqualStefan Kunz
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
Jan 21, 2011·Journal of Virology·Olena ShtankoYoshihiro Kawaoka
Oct 1, 2011·Journal of Virology·Maria Eugenia LoureiroNora Lopez
Feb 22, 2012·Journal of Virology·Shuzo UrataJuan Carlos de la Torre
Sep 13, 2003·Journal of Virology·Rodrigo JácamoMaría T Franze-Fernández
Sep 29, 2004·Journal of Virology·Mar PerezJuan Carlos de la Torre
Nov 27, 2004·Journal of Virology·Meike HassStephan Günther
Mar 3, 2004·Journal of Virology·Tatjana I CornuJuan Carlos de la Torre
Mar 16, 2005·Journal of Virology·Allison GrosethRamon Flick

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.