PMID: 10729120Mar 23, 2000Paper

NP and L proteins of lymphocytic choriomeningitis virus (LCMV) are sufficient for efficient transcription and replication of LCMV genomic RNA analogs

Journal of Virology
K J LeeJuan Carlos de la Torre

Abstract

The genome of lymphocytic choriomeningitis virus (LCMV) consists of two negative-sense single-stranded RNA segments, designated L and S. Both segments contain two viral genes in an ambisense coding strategy, with the genes being separated by an intergenic region (IGR). We have developed a reverse genetic system that allows the investigation of cis-acting signals and trans-acting factors involved in transcription and replication of LCMV. To this end, we constructed an LCMV S minigenome consisting of a negative-sense copy of the chloramphenicol acetyltransferase (CAT) reporter gene flanked upstream by the S 5' untranslated region (UTR) and IGR and downstream by the S 3' UTR. CAT expression was detected in LCMV-infected cells transfected with the minigenome RNA. Intracellular coexpression of the LCMV minigenome and LCMV L and NP proteins supplied from cotransfected plasmids driven by the T7 RNA polymerase provided by the recombinant vaccinia virus vTF7-3 resulted in high levels of CAT activity and synthesis of subgenomic CAT mRNA and antiminigenome RNA species. Thus, L and NP represent the minimal viral trans-acting factors required for efficient RNA synthesis mediated by LCMV polymerase.

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Citations

Apr 5, 2013·Archives of Virology·Frank LennartzWolfgang Garten
Sep 27, 2002·Antiviral Research·C C GarcíaE B Damonte
Jun 5, 2003·Virus Research·Marie Nguyen, Anne-Lise Haenni
Apr 4, 2003·International Journal of Antimicrobial Agents·Cybele C GarcíaElsa B Damonte
Feb 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sebastien F EmonetJuan C de la Torre
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andreas BergthalerDaniel D Pinschewer
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Philip J Kranzusch, Sean P J Whelan
Dec 1, 2010·Antimicrobial Agents and Chemotherapy·Michelle MendenhallBrian B Gowen
Oct 29, 2010·Journal of Virology·Linda BrunotteStephan Günther
Jul 25, 2009·Journal of Virology·Sudhakar S AgnihothramJack H Nunberg
Sep 29, 2006·Journal of Virology·Meike HassStephan Günther
Aug 10, 2012·Journal of Virology·Nicholas L BairdJack H Nunberg
Nov 26, 2010·Journal of Virology·Giulia PasqualStefan Kunz
Dec 17, 2010·Journal of Virology·Jesica M Levingston MacleodNora Lopez
Jan 21, 2011·Journal of Virology·Olena ShtankoYoshihiro Kawaoka
Oct 1, 2011·Journal of Virology·Maria Eugenia LoureiroNora Lopez
Feb 22, 2012·Journal of Virology·Shuzo UrataJuan Carlos de la Torre
Sep 13, 2003·Journal of Virology·Rodrigo JácamoMaría T Franze-Fernández
Sep 29, 2004·Journal of Virology·Mar PerezJuan Carlos de la Torre
Nov 27, 2004·Journal of Virology·Meike HassStephan Günther
Mar 3, 2004·Journal of Virology·Tatjana I CornuJuan Carlos de la Torre
Mar 16, 2005·Journal of Virology·Allison GrosethRamon Flick

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