Abstract
Morphine pretreatment induces ischemic tolerance in neurons, but it remains uncertain whether novel protein kinase C epsilon isoform (nPKCepsilon) and N-methyl-D-aspartate (NMDA) receptors are involved in this neuroprotection. The present study examined this issue. Hippocampal slices from adult BALB/C mice were incubated with morphine at 0.1-10.0 muM in the presence or absence of various antagonists for 30 minutes and then kept in morphine- and antagonist-free buffer for 30 minutes before being subjected to oxygen-glucose deprivation for 20 minutes. After recovery in oxygenated artificial fluid for 5 hours, assessment of slice injury was done by determination of the intensity of slice stain after they were incubated with 2% 2,3,5-triphenyltetrazolium chloride for 30 minutes and extracted by organic solvent for 24 hours. At designated periods, slices were preserved for immunoblot analysis to observe effects of morphine pretreatment on membrane translocation and total protein expression of nPKCepsilon and phosphorylation of NR1 subunits of NMDA receptors. The neuroprotection induced by morphine pretreatment was partially blocked by chelerythrine (a nonselective PKC blocker), epsilonv(1-2) (a selective nPKCepsilon antagonist), MK-...Continue Reading
References
Nov 1, 1986·Stroke; a Journal of Cerebral Circulation·J B BedersonH M Bartkowski
Jul 1, 1988·Neuropathology and Applied Neurobiology·C K ParkG M Teasdale
Sep 22, 1980·Brain Research·I J BakE Morgan
Feb 17, 1983·Nature·A S Kraft, W B Anderson
May 7, 1998·Circulation·J E SchultzG J Gross
Jul 22, 1998·Circulation Research·T SatoE Marbán
Jun 22, 1999·Journal of Neuroscience Research·H M HuangT Hwang
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·G W DornD Mochly-Rosen
Feb 8, 2000·Journal of Neuroscience Methods·E Preston, J Webster
Sep 23, 2000·Journal of Neuroscience Methods·K S MathewsJ A Stamford
Dec 13, 2000·Pharmacology, Biochemistry, and Behavior·A RubajM Sieklucka-Dziuba
Sep 8, 2001·Neurological Research·S OkunoT Sakaki
Sep 13, 2001·Journal of Neurochemistry·H H CheungJ W Gurd
Oct 30, 2001·Neurological Research·K I KatsuraY Katayama
Mar 26, 2002·The Journal of Biological Chemistry·Junfang WuFreesia L Huang
Feb 6, 2004·Journal of Neurochemistry·P E BicklerD M Ferriero
Apr 28, 2004·Anesthesiology·Young Jin LimZhiyi Zuo
Jun 8, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Christian Lange-AsschenfeldtMiguel A Pérez-Pinzón
Citations
Aug 16, 2011·Neurochemical Research·Maria KawalecBarbara Zablocka
Apr 7, 2007·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak, William L Lanier
Mar 20, 2009·Journal of Neurosurgical Anesthesiology·Xu CuiBingxi Zhang
Dec 4, 2009·Journal of Neurosurgical Anesthesiology·Weihua CuiJunfa Li
Oct 6, 2012·Neonatology·Lasse DührsenUrsula Felderhoff-Mueser
Feb 23, 2017·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak, William L Lanier
Mar 20, 2008·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Yan ZhangLong-Chuan Yu
Sep 16, 2015·Journal of Neurosurgical Anesthesiology·Fanjun MengJunfa Li
Dec 7, 2016·Molecular Medicine Reports·Xiaochen GuiTianzuo Li