PMID: 16628065Apr 22, 2006Paper

nPKCepsilon and NMDA receptors participate in neuroprotection induced by morphine pretreatment

Journal of Neurosurgical Anesthesiology
Meng FanjunJi Fang

Abstract

Morphine pretreatment induces ischemic tolerance in neurons, but it remains uncertain whether novel protein kinase C epsilon isoform (nPKCepsilon) and N-methyl-D-aspartate (NMDA) receptors are involved in this neuroprotection. The present study examined this issue. Hippocampal slices from adult BALB/C mice were incubated with morphine at 0.1-10.0 muM in the presence or absence of various antagonists for 30 minutes and then kept in morphine- and antagonist-free buffer for 30 minutes before being subjected to oxygen-glucose deprivation for 20 minutes. After recovery in oxygenated artificial fluid for 5 hours, assessment of slice injury was done by determination of the intensity of slice stain after they were incubated with 2% 2,3,5-triphenyltetrazolium chloride for 30 minutes and extracted by organic solvent for 24 hours. At designated periods, slices were preserved for immunoblot analysis to observe effects of morphine pretreatment on membrane translocation and total protein expression of nPKCepsilon and phosphorylation of NR1 subunits of NMDA receptors. The neuroprotection induced by morphine pretreatment was partially blocked by chelerythrine (a nonselective PKC blocker), epsilonv(1-2) (a selective nPKCepsilon antagonist), MK-...Continue Reading

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Citations

Apr 7, 2007·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak, William L Lanier
Oct 6, 2012·Neonatology·Lasse DührsenUrsula Felderhoff-Mueser
Feb 23, 2017·Journal of Neurosurgical Anesthesiology·Jeffrey J Pasternak, William L Lanier
Mar 20, 2008·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Yan ZhangLong-Chuan Yu

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