Nr0b1 is a negative regulator of Zscan4c in mouse embryonic stem cells

Scientific Reports
Setsuko FujiiH Niwa

Abstract

Nuclear receptor subfamily 0, group B, member 1 (Nr0b1, also known as Dax1) is regarded as an important component of the transcription factor network that governs pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Nr0b1 using the Cre-loxP system and analyzed its precise function. We succeeded in establishing the Nr0b1-null ES cells and confirmed their pluripotency by showing their contribution to chimeric embryos. However, they proliferated slowly with over-expression of 2-cell stage specific transcripts including Zscan4c, which is known to be involved in telomere elongation in ES cells. We revealed that over-expression of Zscan4c prevents normal self-renewal by inducing arrest at G2 phase followed by cell death and that Nr0b1 directly represses the Zscan4c promoter. These data indicated that Nr0b1 is not essential to maintain pluripotency but is involved in the proper activation of 2-cell specific transcripts for self-renewal.

References

Oct 1, 1987·Journal of Cellular Physiology·Y SudaS Aizawa
Feb 15, 1996·Nucleic Acids Research·Y ZhangM Reth
Jul 14, 1998·Nature Biotechnology·F BuchholzA F Stewart
Dec 8, 1998·Nature Genetics·R N YuJ L Jameson
Apr 9, 2002·Genes & Development·Junji FujikuraHitoshi Niwa
Dec 17, 2002·Molecular and Cellular Biology·Taiga SuzukiKazuhiko Umesono
Apr 8, 2003·Nature Genetics·Joshua J MeeksJ Larry Jameson
May 30, 2003·Nature·Kazutoshi TakahashiShinya Yamanaka
Jul 30, 2004·Developmental Biology·Fanyi ZengRichard M Schultz
Jul 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yisong WangYie Liu
Feb 10, 2006·Molecular Genetics and Metabolism·Kathy K NiakanEdward R B McCabe
Sep 15, 2006·Nucleic Acids Research·Wen ZhangTimothy R Hughes
May 13, 2008·Biochemical and Biophysical Research Communications·Chuanhai SunTakashi Yokota
Nov 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Elena P SablinRobert J Fletterick
Jun 17, 2009·Molecular and Cellular Biology·Chuanhai SunTakashi Yokota
Mar 26, 2010·Nature·Michal ZalzmanMinoru S H Ko
Oct 13, 2010·Molecular and Cellular Endocrinology·Victoria R Kelly, Gary D Hammer
Oct 15, 2010·Molecular Endocrinology·Victoria R KellyGary D Hammer
Jun 23, 2012·Nature·Todd S MacfarlanSamuel L Pfaff
Feb 5, 2013·Cell·Cagatay Günes, K Lenhard Rudolph
Mar 20, 2013·Molecular and Cellular Biology·Kousuke UranishiTakashi Yokota
Jun 7, 2013·Nature Communications·Tomokazu AmanoMinoru S H Ko
Aug 8, 2013·BMC Biotechnology·Yuhki NakatakeHitoshi Niwa

❮ Previous
Next ❯

Citations

Jan 20, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Guangming WuHans R Schöler
Aug 21, 2019·Nature Chemical Biology·Young-Chan KimHyo-Soo Kim
Jan 1, 2015·JAK-STAT·Satoshi OhtsukaHitoshi Niwa
Jan 28, 2016·Scientific Reports·Qian ZhangLin Liu
Jan 4, 2017·Nature Reviews. Genetics·Mo Li, Juan Carlos Izpisua Belmonte
Jan 30, 2019·Genes & Development·Mélanie Eckersley-MaslinWolf Reik
Oct 5, 2016·In Vitro Cellular & Developmental Biology. Animal·Kei-Ichiro IshiguroMinoru S H Ko
May 1, 2016·In Vitro Cellular & Developmental Biology. Animal·Lioudmila V SharovaMinoru S H Ko
Oct 28, 2018·Nature Communications·Shin MuraiHiroyasu Nakano
Apr 29, 2021·The EMBO Journal·Daniel OlivieriJoerg Betschinger
May 21, 2021·Reproduction in Domestic Animals = Zuchthygiene·Piyathip SetthawongTheerawat Tharasanit
Aug 3, 2021·Stem Cells International·Jianrong HeRui Jian
Aug 17, 2021·Development·Nicola FestucciaPablo Navarro

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
FACS
genotyping
fluorescence microscopy

Software Mentioned

Flowjo
NIA Array Analysis
MetaMorph

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.