NRAL mediates cisplatin resistance in hepatocellular carcinoma via miR-340-5p/Nrf2 axis

Journal of Cell Communication and Signaling
Li-Li WuLiang Shi

Abstract

Recent studies have shown that long non-coding RNAs (lncRNAs) play a pivotal role in the pathogenesis and progression of hepatocellular carcinoma (HCC). However, the biological action and potential mechanism of liver cancer cell drug resistance have not been clearly clarified. In this study, lncRNAs were screened and differentially expressed in parental and cisplatin-resistant cell lines (HepG2 and HepG2/CDDP). A novel lncRNA, termed NRAL (Nrf2 regulation-associated lncRNA), was identified, and the initial results indicated that it was highly expressed in HepG2 cisplatin resistant cell lines compared to their parental counterparts. Functionally, NRAL depletion significantly enhanced CDDP-mediated cytotoxicity and apoptosis in two cisplatin-resistant HCC cell lines. Mechanistically, the results indicated that NRAL regulates Nrf2 expression through miR-340-5p serving as a competing endogenous RNA (ceRNA), thus influencing the CDDP-induced phenotype in HCC. Collectively, the present investigation suggest that the NRAL/miR-340-5p/Nrf2 axis mediates cisplatin resistance in HCC, which may provide novel targets for overcoming cisplatin resistance in hepatocellular carcinoma cells.

References

Aug 26, 2014·Expert Opinion on Therapeutic Targets·Jun ZhaoMatthew W Lawless
Jan 6, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Liang ShiFang Zheng
Dec 24, 2015·Oxidative Medicine and Cellular Longevity·A L FurfaroM Nitti
Nov 30, 2016·Journal of Translational Medicine·Quan ZhangPatrizia Farci
Mar 10, 2017·Genes·Duncan Ayers, Jo Vandesompele
Mar 16, 2017·World Journal of Gastroenterology : WJG·Alessandro CucchettiVincenzo Mazzaferro

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Citations

Sep 13, 2019·Cell Communication and Signaling : CCS·Jiang-Jiang QinWei-Dong Zhang
Aug 28, 2020·World Journal of Gastroenterology : WJG·Zhao-Shan NiuLi-Mei-Li Tian
Mar 14, 2019·International Journal of Molecular Sciences·Rüdiger Hardeland
Jan 11, 2021·Experimental and Molecular Pathology·Mohammad TaheriMarcel E Dinger
Dec 9, 2020·Cancers·Federico Pio FabrizioLucia Anna Muscarella
Jan 16, 2021·Life Sciences·Ravichandran Jayasuriya, Kunka Mohanram Ramkumar
Mar 9, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Wenxiao JiangXueqiong Zhu
Mar 21, 2021·Free Radical Biology & Medicine·Changhai TianIrving H Zucker
Mar 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sepideh MirzaeiMasoud Najafi

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Methods Mentioned

BETA
immunoprecipitation
RIP
transfection
flow cytometry
pull-down
co-immunoprecipitation

Software Mentioned

Quantity One
Pictar
RNA22
MicroRNA
GraphPad Prism
TargetScan

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