PMID: 8590470Jul 1, 1995Paper

NRE, the major nitrogen regulatory protein of Penicillium chrysogenum, binds specifically to elements in the intergenic promoter regions of nitrate assimilation and penicillin biosynthetic gene clusters

Current Genetics
H Haas, G A Marzluf

Abstract

NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of NRE, the intergenic promoter region of the nitrate reductase/nitrite reductase gene cluster (niiA-niaD) of Penicillium was sequenced. Our results show that NRE is a DNA-binding protein and binds to the intergenic promoter regions of the P. chrysogenum niiA-niaD and acvA-pcbC gene cluster, encoding the first two enzymes in penicillin biosynthesis. Three of the four high-affinity NRE-binding sites contained two GATA core elements. In one of the recognition sites for NRE, one GATA motif was replaced by GATT. The two GATA elements showed all possible orientations, head-to-head, head-to-tail and tail-to-tail, and were separated by between 4 and 27 bp. Missing-contact analysis showed that all three purines in both of the GATA core sequences and the single adenine residue in each of the complementary TATC sequences were involved in the binding of NRE. Moreover, loss of purines in the flanking regions of the GATA elements a...Continue Reading

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Citations

Jun 22, 2011·Applied Microbiology and Biotechnology·Jawad MerhejChristian Barreau
Jun 14, 2005·Phytochemistry·Axel A BrakhagePetra Spröte
Feb 7, 2003·Applied and Environmental Microbiology·Chi-Fai KimRichard Y C Kong
Aug 15, 2014·International Journal of Food Microbiology·Joanna TannousOlivier Puel
Nov 8, 2011·Biotechnology Journal·Stefan S WeberArnold J M Driessen
Jan 25, 2011·Microbial Biotechnology·Juan F MartínCarlos García-Estrada
Jun 30, 2009·Cellular & Molecular Biology Letters·Katherinne NavarreteRenato Chávez
Sep 11, 2012·Fungal Genetics and Biology : FG & B·Rebeca Domínguez-SantosCarlos García-Estrada
Dec 17, 2014·Frontiers in Microbiology·Bettina Tudzynski
Feb 14, 2018·Microbiology and Molecular Biology Reviews : MMBR·Weiping ZhangJian Chen
Nov 23, 2007·Natural Product Reports·J Stefan RokemJens Nielsen
Dec 17, 2020·International Journal of Molecular Sciences·Christelle El Hajj AssafSophie Lorber
Nov 27, 1998·Trends in Biotechnology·M A PeñalvaG Turner
May 29, 2002·Biochimica Et Biophysica Acta·Hung-Chien Roger ChienWen-Hwei Hsu

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