Nrf2 activation attenuates both orthodontic tooth movement and relapse.

Journal of Dental Research
Hiroyuki KanzakiYoshiki Nakamura

Abstract

During orthodontic tooth movement, osteoclasts resorb the alveolar bone at the compress side of periodontium. Reactive oxygen species (ROS) works as intracellular signaling molecules of RANKL during osteoclastogenesis, although ROS has cytotoxicity against cells such as lipid oxidation. To deal with oxidative stress, cells have a defense system that is scavenging ROS by augmented antioxidative stress enzymes via transcriptional regulation with nuclear factor E2-related factor 2 (Nrf2). Previously, we reported that augmented antioxidative stress enzymes by Nrf2-gene transfer inhibited bone destruction. In the present study, we examined the effects of Nrf2 activation on osteoclastogenesis and, thereby, orthodontic tooth movement and orthodontic relapse. Mouse macrophage cell line RAW264.7 cells were used as osteoclast progenitor cells and stimulated with recombinant RANKL (100 ng/mL) with or without Nrf2 activator sulforaphane (SFN) and epigallocatechin gallate (EGCG) or ROS scavenger catechin. Osteoclastogenesis, resorption activity, and osteoclast marker gene expression were examined. Intracellular ROS was analyzed by flow cytometry. Maxillary first molars of C57BL6 male mice were moved palatally with 0.012-inch NiTi wire (100-...Continue Reading

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Citations

Jan 23, 2016·Redox Biology·Hiroyuki KanzakiYoshiki Nakamura
Nov 26, 2015·International Journal of Molecular Sciences·Koji KaidaShunsuke Baba
Oct 1, 2015·The Journal of Nutritional Biochemistry·Ying HuangAh-Ng Tony Kong
Jul 2, 2016·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Maria Cadenas-PerulaAlejandro Iglesias-Linares
Jul 4, 2017·Orthodontics & Craniofacial Research·J PanS Zou
Oct 25, 2017·Journal of Cellular and Molecular Medicine·Yuuki YamaguchiYoshiki Nakamura
Jun 6, 2019·Annual Review of Biomedical Engineering·Nikhil JainViola Vogel
Nov 12, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hiroyuki KanzakiYoshiki Nakamura
Aug 18, 2017·Acta Odontologica Scandinavica·Ling GuanXiaofeng Wang
May 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sophia WedelPidder Jansen-Dürr
Jul 24, 2020·Scientific Reports·Christian KirschneckAgnes Schröder
Nov 26, 2019·Bone Reports·Tsuyoshi NarimiyaHiroshi Tomonari
Feb 12, 2021·Phytotherapy Research : PTR·Marjan TalebiSaeed Samarghandian
Feb 27, 2021·Scientific Reports·John M StarbuckNeus Martínez-Abadías
Apr 17, 2018·Biochemical Pharmacology·María Luisa FerrándizMaria José Alcaraz

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Methods Mentioned

BETA
protein assay
PCR
Nuclear translocation
flow cytometry

Software Mentioned

CanGetSignal
ImageJ
MEPHAS
PrimerBank

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