Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress

International Journal of Molecular Sciences
Yanli LiuYongjin Chen

Abstract

The present study aimed to analyze novel mechanisms underlying Nrf2-mediated anti-apoptosis in periodontal ligament stem cells (PDLSCs) in the periodontitis oxidative microenvironment. We created an oxidative stress model with H₂O₂-treated PDLSCs. We used real-time PCR, Western blotting, TUNEL staining, fluorogenic assay and transfer genetics to confirm the degree of oxidative stress and apoptosis as well as the function of nuclear factor-erythroid 2-related factor 2 (Nrf2). We demonstrated that with upregulated levels of reactive oxygen species (ROS) and malondialdehyde (MDA), the effect of oxidative stress was obvious under H₂O₂ treatment. Oxidative molecules were altered after the H₂O₂ exposure, whereby the signaling of Nrf2 was activated with an increase in its downstream effectors, heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO1) and γ-glutamyl cysteine synthetase (γ-GCS). Additionally, the apoptosis levels gradually increased with oxidative stress by the upregulation of caspase-9, caspase-3, Bax and c-Fos levels in addition to the downregulation of Bcl-2. However, there was no alterations in levels of caspase-8. The enhanced antioxidant effect could not mitigate the occurrence of apoptosis. Furthermore, Nr...Continue Reading

References

Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·P Joseph, A K Jaiswal
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Oct 26, 2000·Nature·P H Krammer
Jun 14, 2003·TheScientificWorldJournal·David J Granville, Roberta A Gottlieb
Apr 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hozumi MotohashiMasayuki Yamamoto
Aug 3, 2004·Science·Douglas R Green, Guido Kroemer
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Jong-Min Lee, Jeffrey A Johnson
Nov 22, 2005·Lancet·Bruce L PihlstromNewell W Johnson
Sep 22, 2006·Antioxidants & Redox Signaling·Cristen PantanoYvonne M W Janssen-Heininger
Dec 5, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin O LeonardCormac T Taylor
Mar 3, 2007·The New England Journal of Medicine·Maurizio S TonettiJohn Deanfield
Jul 20, 2007·PLoS Biology·Guido Kroemer, Klas Blomgren
Nov 3, 2009·Periodontology 2000·Dieter D Bosshardt, Anton Sculean
Jun 10, 2010·Amino Acids·Janusz M GebickiWillem H Koppenol
Aug 27, 2010·Journal of Dental Research·F D'AiutoN Donos
Nov 9, 2010·Biochimica Et Biophysica Acta·Jennefer LindsayAndrew P Gilmore
Nov 26, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Gerasimos P SykiotisDirk Bohmann
Jan 7, 2011·Molecular and Cellular Biochemistry·Mohammad Shamsul OlaHaseeb Ahsan
Jan 22, 2011·Stem Cells and Development·Smit Jayant DangariaThomas G H Diekwisch
Jul 19, 2011·Developmental Cell·Jean-Claude Martinou, Richard J Youle
Oct 14, 2011·Biomaterials·Chan Ho ParkWilliam V Giannobile
Feb 4, 2012·Digestive Diseases and Sciences·Brianna GrigsbyKhalid Khan
Dec 24, 2014·Nature Reviews. Immunology·George Hajishengallis
Apr 11, 2015·Stem Cells International·Wenjun Zhu, Min Liang
Jun 4, 2016·Cell·Nard KubbenTom Misteli
Nov 26, 2016·Genetics and Molecular Research : GMR·Y HeY H Tan

❮ Previous
Next ❯

Citations

May 23, 2018·Clinical Implant Dentistry and Related Research·Gökhan KasnakUlvi Kahraman Gursoy
Oct 3, 2019·Brazilian Oral Research·Belén Retamal-ValdesMagda Feres
Sep 30, 2017·Frontiers in Physiology·Jaya KumarPasuk Mahakknaukrauh
Jan 10, 2019·Oxidative Medicine and Cellular Longevity·Xumin LiShengbin Huang
Aug 28, 2020·Periodontology 2000·Fábio Sá Carneiro SczepanikHoward C Tenenbaum
Jun 6, 2020·Cell Stress & Chaperones·Ines AmaraSalwa Abid-Essefi
Nov 5, 2019·Trends in Molecular Medicine·Xiaozhen DaiYi Tan
Jul 20, 2021·Reports of Biochemistry & Molecular Biology·Latifa Mohamed AbdelgawadMarwa Abdelgwad

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
MDA
transfection
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.