NRF2 Is a Potential Modulator of Hyperresistance to Arsenic Toxicity in Stem-Like Keratinocytes

Oxidative Medicine and Cellular Longevity
Xiafang WuYuanyuan Xu

Abstract

Arsenic is a well-known human carcinogen. Stem cells are indicated to be involved in arsenic carcinogenesis and have a survival selection advantage during arsenic exposure with underlying mechanisms undefined. In the present study, we demonstrated that CD34high-enriched cells derived from HaCaT human keratinocytes showed stem-like phenotypes. These cells were more resistant to arsenic toxicity and had higher arsenic efflux ability than their mature compartments. The master transcription factor in antioxidant defense, nuclear factor erythroid 2-related factor 2 (NRF2) with its downstream genes, was highly expressed in CD34high-enriched cells. Stable knockdown of NRF2 abolished the hyperresistance to arsenic toxicity and holoclone-forming ability of CD34high-enriched cells. Our results suggest that skin epithelial stem/progenitor cells are more resistant to arsenic toxicity than mature cells, which is associated with the high innate expression of NRF2 in skin epithelial stem/progenitor cells.

References

Jan 1, 1993·Chemical Research in Toxicology·N ScottD E Carter
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·M Ramos-GomezT W Kensler
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·K ChanY W Kan
Mar 22, 2003·The Journal of Investigative Dermatology·Carol S TrempusRaymond W Tennant
Nov 5, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jiang LiJeffrey Johnson
Aug 2, 2005·Toxicology and Applied Pharmacology·Timothy J PattersonRobert H Rice
Mar 2, 2006·Molecular Cell·Balasundaram PadmanabhanMasayuki Yamamoto
Aug 5, 2006·Advances in Enzyme Regulation·Makoto Kobayashi, Masayuki Yamamoto
Feb 20, 2007·Toxicology and Applied Pharmacology·Michael P WaalkesBhalchandra A Diwan
Jun 22, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vera S DonnenbergAlbert D Donnenberg
Jun 24, 2008·Free Radical Biology & Medicine·Jingbo PiMichael P Waalkes
Oct 23, 2008·Environmental Science & Technology·Lucie ErbanovaBarbora Dousova
Jan 9, 2010·Environmental Health Perspectives·Erik J TokarMichael P Waalkes
Jan 14, 2010·Journal of the National Cancer Institute·Takashi YorifujiPhilippe Grandjean
Mar 27, 2010·Journal of the National Cancer Institute·Erik J TokarMichael P Waalkes
Jun 11, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Luisa M SolisIgnacio I Wistuba
Aug 19, 2010·Journal of Cellular and Molecular Medicine·Narasimman GurusamyDipak K Das
Sep 2, 2010·Environmental Health Perspectives·Rui ZhaoJingbo Pi
Oct 23, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kyeong-Ah Jung, Mi-Kyoung Kwak
Feb 8, 2011·Cell Stem Cell·Christine E HochmuthHeinrich Jasper
Oct 21, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yang SunMichael P Waalkes
Nov 11, 2011·The Journal of Biological Chemistry·Suryakant K Niture, Anil K Jaiswal
Mar 1, 2012·Cell Stress & Chaperones·Mohammad MohammadzadehMehryar Habibi Roudkenar
Feb 26, 2013·Nature Cell Biology·Jennifer J TsaiMarcel R M van den Brink
Mar 16, 2013·Cell Death & Disease·Kui WangYuquan Wei
Apr 17, 2013·Molecular and Cellular Biology·Alexandria LauDonna D Zhang
Aug 14, 2013·BMC Cancer·Jianhong ZhuMengliang Zhou
Mar 4, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Shohei MurakamiHozumi Motohashi
Apr 23, 2014·Stem Cells·Virve KärkkäinenJari Koistinaho
Jun 21, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xueqian WangDavid S Miller
Aug 12, 2014·The Journal of Pathology·Othman Al-SawafChristoph Jan Wruck

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Citations

Nov 5, 2019·Trends in Molecular Medicine·Xiaozhen DaiYi Tan

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Methods Mentioned

BETA
Flow Cytometry
Assay
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad
Primer
CellQuest
BLAST
FlowJo

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