PMID: 8589408Jul 1, 1995Paper

Nrs1, a repetitive element linked to pisatin demethylase genes on a dispensable chromosome of Nectria haematococca

Molecular Plant-microbe Interactions : MPMI
H G KimH C Kistler

Abstract

We have identified a repetitive DNA element in Nectria haematococca mating population VI, isolate T-2. This repetitive sequence has been called Nrs1. DNA hybridization analysis indicates the sequence is found in several isolates of the fungus pathogenic to Pisum sativum. A 2,027-bp clone containing the Nrs1-2 allele contains a long polyA sequence, imperfect RNA polymerase III promoter sequences, multiple inverted repeats, and the potential for extensive secondary structure similar to known RNA polymerase III transcripts and related retroelements. Ten of the 11 HindIII restriction fragments from isolate T-2 DNA that hybridize to Nrs1-2 segregate in a manner consistent with a 1:1 ratio for random ascospore progeny. The 10 restriction fragment length polymorphism (RFLP) loci define three linkage groups and correspond to three chromosome-sized DNAs from T-2 separated by pulsed field gel electrophoresis. Three RFLP loci defined by hybridization to the gene for pisatin demethylase and localized on the 1.6 million base pair (Mb) chromosome were genetically linked to each other and to several Nrs1 loci. These sequences recombined despite the fact that no obvious homolog exists for the 1.6-Mb chromosome in one parent strain. Allelic RFL...Continue Reading

Citations

Dec 5, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Kempken, U Kück
Nov 10, 2007·Molecular Plant-microbe Interactions : MPMI·Hamid S Garmaroodi, Masatoki Taga
Nov 10, 2001·Annual Review of Phytopathology·U Liane Rosewich, H Corby Kistler
Mar 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alejandro P Rooney, Todd J Ward
Nov 5, 2015·Molecular Plant Pathology·Jeffrey J Coleman
Oct 7, 2003·Annual Review of Microbiology·Marie-Josée Daboussi, Pierre Capy
Jun 24, 1999·Acta Microbiologica Et Immunologica Hungarica·L Hornok
May 24, 2003·Current Genetics·I ChumaS Mayama

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