NS1 interaction with CKII alpha: novel protein complex mediating parvovirus-induced cytotoxicity.

Journal of Virology
J P Nüesch, J Rommelaere

Abstract

During a productive infection, the prototype strain of the parvovirus minute virus of mice (MVMp) induces dramatic morphological alterations in permissive A9 fibroblasts, culminating in cell lysis at the end of infection. These cytopathic effects (CPE) result from rearrangements and destruction of the cytoskeletal micro- and intermediate filaments, while other structures such as the nuclear lamina and particularly the microtubule network remain protected throughout the infection (J. P. F. Nüesch et al., Virology 331:159-174, 2005). In order to unravel the mechanism(s) by which parvoviruses trigger CPE, we searched for NS1 interaction partners by differential affinity chromatography, using distinct NS1 mutants debilitated specifically for this function. Thereby, we isolated an NS1 partner polypeptide, whose interaction with NS1 correlated with the competence of the viral product for CPE induction, and further identified it by tandem mass spectrometry and Western blotting analyses to consist of the catalytic subunit of casein kinase II, CKIIalpha. This interaction of NS1 with CKIIalpha suggested interference by the viral protein with intracellular signaling. Using permanent cell lines expressing dominant-negative CKIIalpha mutant...Continue Reading

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Citations

Oct 12, 2010·Oncogene·V PavetH Gronemeyer
Jan 19, 2008·Cancer Gene Therapy·L KrügerJ A Kleinschmidt
Mar 27, 2009·Journal of Virology·Jürg P F NüeschJean Rommelaere
Jul 16, 2010·Journal of Virology·Annabelle Servant-DelmasSylvie Pillet
Aug 16, 2008·PLoS Pathogens·Séverine BärJürg P F Nüesch
Jun 23, 2010·International Journal of Medical Sciences·Violetta KivovichStanley J Naides
Feb 1, 2011·International Journal of Medical Sciences·Brian D PooleStanley J Naides
Feb 19, 2011·Future Virology·Aaron Yun Chen, Jianming Qiu
Jul 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jürg P F Nüesch, Jean Rommelaere
Mar 10, 2016·Annual Review of Virology·Shweta KailasanColin R Parrish
Aug 5, 2015·Virology Journal·Mengyu TuAnchun Cheng
Nov 20, 2014·Cellular and Molecular Life Sciences : CMLS·Shishir Kumar GuptaA K Tiwari
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shishir Kumar GuptaA P Sahoo
Jan 24, 2017·Veterinary Microbiology·Xiaomei ZhangWeiquan Liu
Oct 10, 2007·Journal of Hepatology·Francesca Dal PeroMartina Gerotto
Oct 20, 2009·European Journal of Pharmacology·Mathieu H M Noteborn
Aug 11, 2017·Human Gene Therapy·Margaret R DuffyLen W Seymour
Jul 13, 2012·Journal of Virology·Nazim El-AndaloussiAntonio Marchini
May 9, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jürg P F NüeschJean Rommelaere
Jan 22, 2021·Journal of Virology·Mengxiao LuoMavis Agbandje-McKenna
Apr 25, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dominik WitzigmannJörg Huwyler

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