Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae.

Nucleic Acids Research
Cheol Woong HaWon-Ki Huh

Abstract

In eukaryotic cells, ribosomal DNA (rDNA) forms the basis of the nucleolus. In Saccharomyces cerevisiae, 100-200 copies of a 9.1-kb rDNA repeat exist as a tandem array on chromosome XII. The stability of this highly repetitive array is maintained through silencing. However, the precise mechanisms that regulate rDNA silencing are poorly understood. Here, we report that S. cerevisiae Ydr026c, which we name NTS1 silencing protein 1 (Nsi1), plays a significant role in rDNA silencing. By studying the subcellular localization of 159 nucleolar proteins, we identified 11 proteins whose localization pattern is similar to that of Net1, a well-established rDNA silencing factor. Among these proteins is Nsi1, which is associated with the NTS1 region of rDNA and is required for rDNA silencing at NTS1. In addition, Nsi1 physically interacts with the known rDNA silencing factors Net1, Sir2 and Fob1. The loss of Nsi1 decreases the association of Sir2 with NTS1 and increases histone acetylation at NTS1. Furthermore, Nsi1 contributes to the longevity of yeast cells. Taken together, our findings suggest that Nsi1 is a new rDNA silencing factor that contributes to rDNA stability and lifespan extension in S. cerevisiae.

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Citations

Oct 25, 2012·Biochimica Et Biophysica Acta·Attila NémethHerbert Tschochner
Jul 19, 2012·The EMBO Journal·Alarich ReiterHerbert Tschochner
Apr 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Rahul JaiswalCarlos R Escalante
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Jan 1, 2014·FEMS Yeast Research·Austen R D Ganley, Takehiko Kobayashi
Jul 3, 2016·Trends in Genetics : TIG·Odil PorruaDomenico Libri

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Methods Mentioned

BETA
PCR
ChIP
immunoprecipitation
pull-down
environmental stresses
fluorescence microscopy
CoIP
acetylation
histone acetylation
electrophoresis

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