NsrR from Streptomyces coelicolor is a nitric oxide-sensing [4Fe-4S] cluster protein with a specialized regulatory function

The Journal of Biological Chemistry
Jason C CrackNick E Le Brun

Abstract

The Rrf2 family transcription factor NsrR controls expression of genes in a wide range of bacteria in response to nitric oxide (NO). The precise form of the NO-sensing module of NsrR is the subject of controversy because NsrR proteins containing either [2Fe-2S] or [4Fe-4S] clusters have been observed previously. Optical, Mössbauer, resonance Raman spectroscopies and native mass spectrometry demonstrate that Streptomyces coelicolor NsrR (ScNsrR), previously reported to contain a [2Fe-2S] cluster, can be isolated containing a [4Fe-4S] cluster. ChIP-seq experiments indicated that the ScNsrR regulon is small, consisting of only hmpA1, hmpA2, and nsrR itself. The hmpA genes encode NO-detoxifying flavohemoglobins, indicating that ScNsrR has a specialized regulatory function focused on NO detoxification and is not a global regulator like some NsrR orthologues. EMSAs and DNase I footprinting showed that the [4Fe-4S] form of ScNsrR binds specifically and tightly to an 11-bp inverted repeat sequence in the promoter regions of the identified target genes and that DNA binding is abolished following reaction with NO. Resonance Raman data were consistent with cluster coordination by three Cys residues and one oxygen-containing residue, and a...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 1, 1983·Methods in Enzymology·P W RiddlesB Zerner
May 1, 1993·Journal of Bacteriology·G L NewtonY Aharonowitz
Aug 1, 1997·Science·H BeinertEckard Münck
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·C J SchwartzPatricia J Kiley
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertolt GustKeith F Chater
Aug 19, 2003·Journal of Bacteriology·Matthew A GregoryMargaret C M Smith
May 7, 2004·Nature·Johan A KersRosemary Loria
Sep 15, 2004·Biochemistry·Robert M CicchilloSquire J Booker
Feb 22, 2005·Molecular Aspects of Medicine·Richard Bruckdorfer
Sep 30, 2005·Nature·Benoît D'AutréauxStephen Spiro
Nov 2, 2005·PLoS Computational Biology·Dmitry A RodionovMikhail S Gelfand
Aug 4, 2006·Journal of Bacteriology·Michiko M NakanoKazuo Kobayashi
Apr 25, 2007·Nucleic Acids Research·Warren A Kibbe
Jan 5, 2008·Organic & Biomolecular Chemistry·Matthew J G StewartChris J Hamilton
Jan 12, 2008·Journal of the American Chemical Society·Jason C CrackAndrew J Thomson
Nov 15, 2008·Molecular Microbiology·Vincent M IsabellaVirginia L Clark
Nov 26, 2008·Natural Product Reports·Vishnu Karthik Jothivasan, Chris J Hamilton
Apr 21, 2009·Trends in Microbiology·Jawahar Sudhamsu, Brian R Crane
Jun 30, 2009·Journal of Bacteriology·Young-Man Kwon, Bernard Weiss
Feb 20, 2010·Trends in Microbiology·Nicholas P TuckerMatthew I Hutchings
Dec 25, 2010·Journal of the American Chemical Society·Jason C CrackNick E Le Brun
Oct 28, 2011·FEMS Microbiology Letters·Claire E Vine, Jeffrey A Cole
Dec 8, 2011·The Journal of Biological Chemistry·Douglas T Hess, Jonathan S Stamler
Mar 6, 2012·Current Opinion in Chemical Biology·Jason C CrackNick E Le Brun
Apr 28, 2012·Science·Divya SethJonathan S Stamler
May 16, 2012·Biochemistry·Angela S FleischhackerPatricia J Kiley
Jul 27, 2012·Molecular Microbiology·Joyce E KarlinseyFerric C Fang
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bo ZhangMichael K Johnson
Mar 9, 2013·The Journal of Biological Chemistry·Jason C CrackNick E Le Brun
May 7, 2013·Nature Structural & Molecular Biology·Senapathy RajagopalanPatricia J Kiley
Mar 19, 2014·Methods in Molecular Biology·Jason C CrackNick E Le Brun
Aug 8, 2014·PLoS Genetics·Mahmoud M Al-BassamMark J Buttner

Citations

Jan 23, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jason C CrackNick E Le Brun
Mar 22, 2016·Applied Microbiology and Biotechnology·Jung Chul ParkHeung-Shick Lee
Aug 12, 2016·Cell Host & Microbe·Ferric C FangAndrés Vázquez-Torres
Aug 20, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Christine WachnowskyJ A Cowan
Nov 2, 2016·The Plant Journal : for Cell and Molecular Biology·Emma L BastowJanneke Balk
Apr 21, 2017·Nature Communications·Anne VolbedaJuan C Fontecilla-Camps
Jun 3, 2015·Methods : a Companion to Methods in Enzymology·Kevin S MyersPatricia J Kiley
Oct 3, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Apr 6, 2018·Chemical Science·Ma Teresa Pellicer MartinezNick E Le Brun
Dec 9, 2017·Antioxidants & Redox Signaling·Jason C Crack, Nick E Le Brun
Jan 26, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Smilja Todorovic, Miguel Teixeira
Aug 24, 2018·Metallomics : Integrated Biometal Science·Sambuddha SenJ A Cowan
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Sep 5, 2018·Molecular Microbiology·Matthew J Bush
Dec 19, 2016·F1000Research·Keith F Chater
Sep 19, 2019·ELife·Ma Teresa Pellicer MartinezNick E Le Brun
Jan 14, 2017·The Journal of Biological Chemistry·Zishuo ChengHuangen Ding
May 4, 2020·Biotechnology Journal·Irisappan GaneshJeong Wook Lee
Apr 19, 2020·The Journal of Biological Chemistry·Melissa Y Y StewartNick E Le Brun
Sep 8, 2018·Biochemical Society Transactions·Lauren K WarehamRobert K Poole
May 24, 2020·Scientific Reports·Aaron Millan-OropezaMarie-Joelle Virolle
Apr 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jason C CrackNick E Le Brun
Sep 10, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Daniel B GrabarczykKylie A Vincent
Jan 22, 2019·Biology Methods and Protocols·Jason C CrackNick E Le Brun
Feb 19, 2016·The Journal of Biological Chemistry·Jason C CrackNick E Le Brun
Aug 12, 2020·Journal of Industrial Microbiology & Biotechnology·Yongjae LeeByung-Kwan Cho

Related Concepts

Bacterial Proteins
DNA Helix Destabilizing Proteins
Iron-Sulfur Proteins
Nitric Oxide
Early Promoters, Genetic
Regulon
Streptomyces coelicolor A3(2)
Analogs & derivatives
Cysteine
Deoxyribonuclease I

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