NT2 derived neuronal and astrocytic network signalling.

PloS One
E J HillH Rheinallt Parri

Abstract

A major focus of stem cell research is the generation of neurons that may then be implanted to treat neurodegenerative diseases. However, a picture is emerging where astrocytes are partners to neurons in sustaining and modulating brain function. We therefore investigated the functional properties of NT2 derived astrocytes and neurons using electrophysiological and calcium imaging approaches. NT2 neurons (NT2Ns) expressed sodium dependent action potentials, as well as responses to depolarisation and the neurotransmitter glutamate. NT2Ns exhibited spontaneous and coordinated calcium elevations in clusters and in extended processes, indicating local and long distance signalling. Tetrodotoxin sensitive network activity could also be evoked by electrical stimulation. Similarly, NT2 astrocytes (NT2As) exhibited morphology and functional properties consistent with this glial cell type. NT2As responded to neuronal activity and to exogenously applied neurotransmitters with calcium elevations, and in contrast to neurons, also exhibited spontaneous rhythmic calcium oscillations. NT2As also generated propagating calcium waves that were gap junction and purinergic signalling dependent. Our results show that NT2 derived astrocytes exhibit ap...Continue Reading

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Citations

Apr 9, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marta A TarczylukEric J Hill
May 30, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marta A TarczylukEric J Hill
Feb 26, 2015·Journal of Tissue Engineering and Regenerative Medicine·Imogen SmithBenjamin J Whalley
Dec 5, 2015·The Journal of Physiology·Eric HillMichael Coleman
Mar 16, 2017·Journal of Neural Engineering·B J RaosC P Unsworth
May 7, 2020·RNA Biology·Charannya Sozheesvari SubhramanyamQidong Hu
Feb 12, 2014·Alternatives to Laboratory Animals : ATLA·Elizabeth K WoehrlingMichael D Coleman
Sep 21, 2018·Frontiers in Neuroscience·Alastair I GraingerEric J Hill
Feb 19, 2021·Journal of Neural Engineering·Si LiCharles P Unsworth

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Methods Mentioned

BETA
fluorescence imaging
mechanical dissociation

Software Mentioned

Sigmaplot
pClamp
Clampfit
Simple PCI

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